A new electrophoretic method for determining ligand: Technetium stoichiometry in carrier free 99mTc-radiopharmaceuticals

L. G. Marzilli, P. Worley, H. D. Burns

Research output: Contribution to journalArticle

Abstract

An electrophoretic procedure is outlined for the determination of the number of ligands bound to technetium-99m radiopharmaceuticals. The approach involves use of ligands that will complex technetium in a similar fashion but that differ in charge. This approach was applied experimentally to dimercapto ligands in which the ligating sulfur atoms are separated by a flexible three-carbon chain (1,3-dimercapto compounds). Two such ligands studied are 1,3-dimercaptopropane (DMP) and dihydrothioctic acid (DHTA). The Tc compound of DHTA migrates much farther on electrophoresis than the Tc complex of DMP. However, when TcO4- is reduced by SnCl2 or NaBH4 in the presence of equimolar quantities of DHTA and DMP, a new compound is formed being twice as abundant as either the TcDMP or the TcDHTA compound and migrating an intermediate distance. The formation of this new complex and the 1:2:1 distribution indicates that two 1,3-dimercapto compounds are attached to the Tc-center in all three compounds.

Original languageEnglish (US)
Pages (from-to)871-876
Number of pages6
JournalJournal of Nuclear Medicine
Volume20
Issue number8
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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