A neural network classifier capable of recognizing the patterns of all major subcellular structures in fluorescence microscope images of HeLa cells

M. V. Boland, R. F. Murphy

Research output: Contribution to journalArticlepeer-review

Abstract

Motivation: Assessment of protein subcellular location is crucial to proteomics efforts since localization information provides a context for a protein's sequence, structure, and function. The work described below is the first to address the subcellular localization of proteins in a quantitative, comprehensive manner. Results: Images for ten different subcellular patterns (including all major organelles) were collected using fluorescence microscopy. The patterns were described using a variety of numeric features, including Zernike moments, Haralick texture features, and a set of new features developed specifically for this purpose. To test the usefulness of these features, they were used to train a neural network classifier. The classifier was able to correctly recognize an average of 83% of previously unseen cells showing one of the ten patterns. The same classifier was then used to recognize previously unseen sets of homogeneously prepared cells with 98% accuracy.

Original languageEnglish (US)
Pages (from-to)1213-1223
Number of pages11
JournalBioinformatics
Volume17
Issue number12
DOIs
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Statistics and Probability
  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Computational Theory and Mathematics
  • Computational Mathematics

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