A net mold-based method of scaffold-free three-dimensional cardiac tissue creation

Yang Bai, Enoch Yeung, Cecillia Lui, Chin Siang Ong, Isaree Pitaktong, Chenyu Huang, Takahiro Inoue, Hiroshi Matsushita, Chunye Ma, Narutoshi Hibino

Research output: Contribution to journalArticle

Abstract

This protocol describes a novel and easy net mold-based method to create three-dimensional (3-D) cardiac tissues without additional scaffold material. Human-induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs), human cardiac fibroblasts (HCFs), and human umbilical vein endothelial cells (HUVECs) are isolated and used to generate a cell suspension with 70% iPSC-CMs, 15% HCFs, and 15% HUVECs. They are co-cultured in an ultra-low attachment "hanging drop" system, which contains micropores for condensing hundreds of spheroids at one time. The cells aggregate and spontaneously form beating spheroids after 3 days of co-culture. The spheroids are harvested, seeded into a novel mold cavity, and cultured on a shaker in the incubator. The spheroids become a mature functional tissue approximately 7 days after seeding. The resultant multilayered tissues consist of fused spheroids with satisfactory structural integrity and synchronous beating behavior. This new method has promising potential as a reproducible and cost-effective method to create engineered tissues for the treatment of heart failure in the future.

Original languageEnglish (US)
Article numbere58252
JournalJournal of Visualized Experiments
Volume2018
Issue number138
DOIs
StatePublished - Aug 5 2018

Fingerprint

Scaffolds
Fungi
Tissue
Induced Pluripotent Stem Cells
Endothelial cells
Human Umbilical Vein Endothelial Cells
Fibroblasts
Stem cells
Cardiac Myocytes
Incubators
Structural integrity
Coculture Techniques
Treatment Failure
Suspensions
Heart Failure
Costs and Cost Analysis
Costs

Keywords

  • Bioengineering
  • Cardiac tissue engineering
  • Hanging drop
  • Heart failure
  • Issue 138
  • Net-mold
  • Scaffold-free
  • Spheroids

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Bai, Y., Yeung, E., Lui, C., Ong, C. S., Pitaktong, I., Huang, C., ... Hibino, N. (2018). A net mold-based method of scaffold-free three-dimensional cardiac tissue creation. Journal of Visualized Experiments, 2018(138), [e58252]. https://doi.org/10.3791/58252

A net mold-based method of scaffold-free three-dimensional cardiac tissue creation. / Bai, Yang; Yeung, Enoch; Lui, Cecillia; Ong, Chin Siang; Pitaktong, Isaree; Huang, Chenyu; Inoue, Takahiro; Matsushita, Hiroshi; Ma, Chunye; Hibino, Narutoshi.

In: Journal of Visualized Experiments, Vol. 2018, No. 138, e58252, 05.08.2018.

Research output: Contribution to journalArticle

Bai, Y, Yeung, E, Lui, C, Ong, CS, Pitaktong, I, Huang, C, Inoue, T, Matsushita, H, Ma, C & Hibino, N 2018, 'A net mold-based method of scaffold-free three-dimensional cardiac tissue creation', Journal of Visualized Experiments, vol. 2018, no. 138, e58252. https://doi.org/10.3791/58252
Bai, Yang ; Yeung, Enoch ; Lui, Cecillia ; Ong, Chin Siang ; Pitaktong, Isaree ; Huang, Chenyu ; Inoue, Takahiro ; Matsushita, Hiroshi ; Ma, Chunye ; Hibino, Narutoshi. / A net mold-based method of scaffold-free three-dimensional cardiac tissue creation. In: Journal of Visualized Experiments. 2018 ; Vol. 2018, No. 138.
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