A multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase a dynamics in parallel

Nwe Nwe Aye-Han, Jin Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Parallel detection of signaling activities allows us to correlate activity dynamics between signaling molecules. In this review, we detail a multiparameter live cell imaging method to monitor 3′,5′-cyclic adenosine monophosphate (cAMP) levels and protein kinase A (PKA) activities in parallel.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages207-215
Number of pages9
Volume1071
ISBN (Print)9781627036214
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1071
ISSN (Print)10643745

Keywords

  • cAMP
  • FRET
  • Parallel imaging
  • Protein kinase A
  • Signal transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Aye-Han, N. N., & Zhang, J. (2014). A multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase a dynamics in parallel. In Methods in Molecular Biology (Vol. 1071, pp. 207-215). (Methods in Molecular Biology; Vol. 1071). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-622-1_16