A mouse model of proliferative vitreoretinopathy induced by dispase

M. Valeria Cantó Soler, Juan E. Gallo, Ricardo A. Dodds, Angela M. Suburo

Research output: Contribution to journalArticle

Abstract

A proliferative vitreoretinopathy-like condition induced by intravitreal dispase injection in C57BL/6J mice was studied using ophthalmoscopic and histochemical procedures. The frequency of intravitreal hemorrhage, intravitreal spots, retinal folds and epiretinal membranes was scored by ophthalmoscopic examination at 1, 2, 4, 6 and 8 weeks after the injection. Intravitreal spots corresponded to free cells exhibiting F4/80 immunoreactivity, a macrophage/microglial marker. Retinal folds always appeared before an epiretinal membrane could be observed. Dispase-injected eyes always showed a much higher frequency of folds and membranes than saline-injected eyes. Folds and membranes appeared earlier and were more extensive in the presence of intravitreal hemorrhage than in its absence. Müller retinal cells exhibited significant changes in glial fibrillary acidic protein-immunoreactivity. This was absent in normal Müller cells but, in dispase-injected animals, it was expressed in radial processes at the site of retinal folds, later extending to the whole retina. Both epi- and subretinal membranes contained cells probably derived from Müller cells, since they exhibited co-localization of glial fibrillary acidic protein- and glutamine synthase immunoreactivities. F4/80 was also present in numerous cells within the retina, epi- and subretinal membranes. By contrast, the retinal pigment epithelium cell marker RPE65 was restricted to subretinal membranes. It can be concluded that dispase induced a proliferative vitreoretinopathy-like condition in mice, with a strong contribution of macrophage- and glial-derived cells.

Original languageEnglish (US)
Pages (from-to)491-504
Number of pages14
JournalExperimental Eye Research
Volume75
Issue number5
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Proliferative Vitreoretinopathy
Epiretinal Membrane
Glial Fibrillary Acidic Protein
Membranes
Retina
Macrophages
Hemorrhage
Intravitreal Injections
Retinal Pigment Epithelium
dispase
Glutamine
Inbred C57BL Mouse
Neuroglia
Injections

Keywords

  • Animal model
  • Dispase
  • Epiretinal membrane
  • F4/80
  • Glial fibrillary acidic protein
  • Glutamine synthase
  • Macrophage
  • Mice
  • Microglia
  • Müller glial cell
  • Proliferative vitreoretinopathy
  • Retinal detachment
  • RPE65
  • Vitreal hemorrhage

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Cantó Soler, M. V., Gallo, J. E., Dodds, R. A., & Suburo, A. M. (2002). A mouse model of proliferative vitreoretinopathy induced by dispase. Experimental Eye Research, 75(5), 491-504. https://doi.org/10.1006/exer.2002.2031

A mouse model of proliferative vitreoretinopathy induced by dispase. / Cantó Soler, M. Valeria; Gallo, Juan E.; Dodds, Ricardo A.; Suburo, Angela M.

In: Experimental Eye Research, Vol. 75, No. 5, 2002, p. 491-504.

Research output: Contribution to journalArticle

Cantó Soler, MV, Gallo, JE, Dodds, RA & Suburo, AM 2002, 'A mouse model of proliferative vitreoretinopathy induced by dispase', Experimental Eye Research, vol. 75, no. 5, pp. 491-504. https://doi.org/10.1006/exer.2002.2031
Cantó Soler, M. Valeria ; Gallo, Juan E. ; Dodds, Ricardo A. ; Suburo, Angela M. / A mouse model of proliferative vitreoretinopathy induced by dispase. In: Experimental Eye Research. 2002 ; Vol. 75, No. 5. pp. 491-504.
@article{6912f385212e4a8c92a76d884e33937a,
title = "A mouse model of proliferative vitreoretinopathy induced by dispase",
abstract = "A proliferative vitreoretinopathy-like condition induced by intravitreal dispase injection in C57BL/6J mice was studied using ophthalmoscopic and histochemical procedures. The frequency of intravitreal hemorrhage, intravitreal spots, retinal folds and epiretinal membranes was scored by ophthalmoscopic examination at 1, 2, 4, 6 and 8 weeks after the injection. Intravitreal spots corresponded to free cells exhibiting F4/80 immunoreactivity, a macrophage/microglial marker. Retinal folds always appeared before an epiretinal membrane could be observed. Dispase-injected eyes always showed a much higher frequency of folds and membranes than saline-injected eyes. Folds and membranes appeared earlier and were more extensive in the presence of intravitreal hemorrhage than in its absence. M{\"u}ller retinal cells exhibited significant changes in glial fibrillary acidic protein-immunoreactivity. This was absent in normal M{\"u}ller cells but, in dispase-injected animals, it was expressed in radial processes at the site of retinal folds, later extending to the whole retina. Both epi- and subretinal membranes contained cells probably derived from M{\"u}ller cells, since they exhibited co-localization of glial fibrillary acidic protein- and glutamine synthase immunoreactivities. F4/80 was also present in numerous cells within the retina, epi- and subretinal membranes. By contrast, the retinal pigment epithelium cell marker RPE65 was restricted to subretinal membranes. It can be concluded that dispase induced a proliferative vitreoretinopathy-like condition in mice, with a strong contribution of macrophage- and glial-derived cells.",
keywords = "Animal model, Dispase, Epiretinal membrane, F4/80, Glial fibrillary acidic protein, Glutamine synthase, Macrophage, Mice, Microglia, M{\"u}ller glial cell, Proliferative vitreoretinopathy, Retinal detachment, RPE65, Vitreal hemorrhage",
author = "{Cant{\'o} Soler}, {M. Valeria} and Gallo, {Juan E.} and Dodds, {Ricardo A.} and Suburo, {Angela M.}",
year = "2002",
doi = "10.1006/exer.2002.2031",
language = "English (US)",
volume = "75",
pages = "491--504",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - A mouse model of proliferative vitreoretinopathy induced by dispase

AU - Cantó Soler, M. Valeria

AU - Gallo, Juan E.

AU - Dodds, Ricardo A.

AU - Suburo, Angela M.

PY - 2002

Y1 - 2002

N2 - A proliferative vitreoretinopathy-like condition induced by intravitreal dispase injection in C57BL/6J mice was studied using ophthalmoscopic and histochemical procedures. The frequency of intravitreal hemorrhage, intravitreal spots, retinal folds and epiretinal membranes was scored by ophthalmoscopic examination at 1, 2, 4, 6 and 8 weeks after the injection. Intravitreal spots corresponded to free cells exhibiting F4/80 immunoreactivity, a macrophage/microglial marker. Retinal folds always appeared before an epiretinal membrane could be observed. Dispase-injected eyes always showed a much higher frequency of folds and membranes than saline-injected eyes. Folds and membranes appeared earlier and were more extensive in the presence of intravitreal hemorrhage than in its absence. Müller retinal cells exhibited significant changes in glial fibrillary acidic protein-immunoreactivity. This was absent in normal Müller cells but, in dispase-injected animals, it was expressed in radial processes at the site of retinal folds, later extending to the whole retina. Both epi- and subretinal membranes contained cells probably derived from Müller cells, since they exhibited co-localization of glial fibrillary acidic protein- and glutamine synthase immunoreactivities. F4/80 was also present in numerous cells within the retina, epi- and subretinal membranes. By contrast, the retinal pigment epithelium cell marker RPE65 was restricted to subretinal membranes. It can be concluded that dispase induced a proliferative vitreoretinopathy-like condition in mice, with a strong contribution of macrophage- and glial-derived cells.

AB - A proliferative vitreoretinopathy-like condition induced by intravitreal dispase injection in C57BL/6J mice was studied using ophthalmoscopic and histochemical procedures. The frequency of intravitreal hemorrhage, intravitreal spots, retinal folds and epiretinal membranes was scored by ophthalmoscopic examination at 1, 2, 4, 6 and 8 weeks after the injection. Intravitreal spots corresponded to free cells exhibiting F4/80 immunoreactivity, a macrophage/microglial marker. Retinal folds always appeared before an epiretinal membrane could be observed. Dispase-injected eyes always showed a much higher frequency of folds and membranes than saline-injected eyes. Folds and membranes appeared earlier and were more extensive in the presence of intravitreal hemorrhage than in its absence. Müller retinal cells exhibited significant changes in glial fibrillary acidic protein-immunoreactivity. This was absent in normal Müller cells but, in dispase-injected animals, it was expressed in radial processes at the site of retinal folds, later extending to the whole retina. Both epi- and subretinal membranes contained cells probably derived from Müller cells, since they exhibited co-localization of glial fibrillary acidic protein- and glutamine synthase immunoreactivities. F4/80 was also present in numerous cells within the retina, epi- and subretinal membranes. By contrast, the retinal pigment epithelium cell marker RPE65 was restricted to subretinal membranes. It can be concluded that dispase induced a proliferative vitreoretinopathy-like condition in mice, with a strong contribution of macrophage- and glial-derived cells.

KW - Animal model

KW - Dispase

KW - Epiretinal membrane

KW - F4/80

KW - Glial fibrillary acidic protein

KW - Glutamine synthase

KW - Macrophage

KW - Mice

KW - Microglia

KW - Müller glial cell

KW - Proliferative vitreoretinopathy

KW - Retinal detachment

KW - RPE65

KW - Vitreal hemorrhage

UR - http://www.scopus.com/inward/record.url?scp=0036451197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036451197&partnerID=8YFLogxK

U2 - 10.1006/exer.2002.2031

DO - 10.1006/exer.2002.2031

M3 - Article

C2 - 12457862

AN - SCOPUS:0036451197

VL - 75

SP - 491

EP - 504

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 5

ER -