TY - JOUR
T1 - A mouse model of lumbar spine instability
AU - Liu, Shufen
AU - Sun, Yueli
AU - Dong, Jingcheng
AU - Bian, Qin
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (81973607) and Essential Drug Research and Development (2019ZX09201004-003-032) from Ministry of Science and Technology of China.
Publisher Copyright:
© 2021 JoVE Journal of Visualized Experiments.
PY - 2021/4
Y1 - 2021/4
N2 - Intervertebral disc degeneration (IDD) is a common pathological change leading to low back pain. Appropriate animal models are desired for understanding the pathological processes and evaluating new drugs. Here, we introduced a surgically induced lumbar spine instability (LSI) mouse model that develops IDD starting from 1 week post operation. In detail, the mouse under anesthesia was operated by low back skin incision, L3–L5 spinous processes exposure, detachment of paraspinous muscles, resection of processes and ligaments, and skin closure. L4–L5 IVDs were chosen for the observation. The LSI model develops lumbar IDD by porosity and hypertrophy in endplates at an early stage, decrease in intervertebral disc volume, shrinkage in nucleus pulposus at an intermediate stage, and bone loss in lumbar vertebrae (L5) at a later stage. The LSI mouse model has the advantages of strong operability, no requirement of special equipment, reproducibility, inexpensive, and relatively short period of IDD development. However, LSI operation is still a trauma that causes inflammation within the first week post operation. Thus, this animal model is suitable for study of lumbar IDD.
AB - Intervertebral disc degeneration (IDD) is a common pathological change leading to low back pain. Appropriate animal models are desired for understanding the pathological processes and evaluating new drugs. Here, we introduced a surgically induced lumbar spine instability (LSI) mouse model that develops IDD starting from 1 week post operation. In detail, the mouse under anesthesia was operated by low back skin incision, L3–L5 spinous processes exposure, detachment of paraspinous muscles, resection of processes and ligaments, and skin closure. L4–L5 IVDs were chosen for the observation. The LSI model develops lumbar IDD by porosity and hypertrophy in endplates at an early stage, decrease in intervertebral disc volume, shrinkage in nucleus pulposus at an intermediate stage, and bone loss in lumbar vertebrae (L5) at a later stage. The LSI mouse model has the advantages of strong operability, no requirement of special equipment, reproducibility, inexpensive, and relatively short period of IDD development. However, LSI operation is still a trauma that causes inflammation within the first week post operation. Thus, this animal model is suitable for study of lumbar IDD.
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U2 - 10.3791/61722
DO - 10.3791/61722
M3 - Article
C2 - 33970135
AN - SCOPUS:85105704117
SN - 1940-087X
VL - 2021
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 170
M1 - e61722
ER -