A mouse model of albright hereditary osteodystrophy generated by targeted disruption of exon 1 of the Gnas gene

Emily L. Germain-Lee, William Schwindinger, Janet Crane, Rediet Zewdu, Larry S. Zweifel, Gary S Wand, David L. Huso, Motoyasu Saji, Matthew D. Ringel, Michael A. Levine

Research output: Contribution to journalArticle

Abstract

Albright hereditary osteodystrophy is caused by heterozygous inactivating mutations in GNAS, a gene that encodes not only the α-chain of Gs (Gαs), but also NESP55 and XLαs through use of alternative first exons. Patients with GNAS mutations on maternally inherited alleles are resistant to multiple hormones such as PTH, TSH, LH/FSH, GHRH, and glucagon, whose receptors are coupled to Gs. This variant of Albright hereditary osteodystrophy is termed pseudohypoparathyroidism type 1a and is due to presumed tissue-specific paternal imprinting of Gαs. Previous studies have shown that mice heterozygous for a targeted disruption of exon 2 of Gnas, the murine homolog of GNAS, showed unique phenotypes dependent on the parent of origin of the mutated allele. However, hormone resistance occurred only when the disrupted gene was maternally inherited. Because disruption of exon 2 is predicted to inactivate Gαs as well as NESP55 and XLαs, we created transgenic mice with disruption of exon 1 to investigate the effects of isolated loss of Gαs. Heterozygous mice that inherited the disruption maternally (-m/+) exhibited PTH and TSH resistance, whereas those with paternal inheritance (+/-p) had normal hormone responsiveness. Heterozygous mice were shorter and, when the disrupted allele was inherited maternally, weighed more than wild-type littermates. Gαs protein and mRNA expression was consistent with paternal imprinting in the renal cortex and thyroid, but there was no imprinting in renal medulla, heart, or adipose. These findings confirm the tissue-specific paternal imprinting of GNAS and demonstrate that Gαs deficiency alone is sufficient to account for the hormone resistance of pseudohypoparathyroidism type 1a.

Original languageEnglish (US)
Pages (from-to)4697-4709
Number of pages13
JournalEndocrinology
Volume146
Issue number11
DOIs
StatePublished - Nov 2005

Fingerprint

Exons
Pseudohypoparathyroidism
Hormones
Alleles
Genes
Glucagon Receptors
Kidney
Mutation
Transgenic Mice
Thyroid Gland
Phenotype
Messenger RNA
Maternal Inheritance
Albright's hereditary osteodystrophy
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

A mouse model of albright hereditary osteodystrophy generated by targeted disruption of exon 1 of the Gnas gene. / Germain-Lee, Emily L.; Schwindinger, William; Crane, Janet; Zewdu, Rediet; Zweifel, Larry S.; Wand, Gary S; Huso, David L.; Saji, Motoyasu; Ringel, Matthew D.; Levine, Michael A.

In: Endocrinology, Vol. 146, No. 11, 11.2005, p. 4697-4709.

Research output: Contribution to journalArticle

Germain-Lee, EL, Schwindinger, W, Crane, J, Zewdu, R, Zweifel, LS, Wand, GS, Huso, DL, Saji, M, Ringel, MD & Levine, MA 2005, 'A mouse model of albright hereditary osteodystrophy generated by targeted disruption of exon 1 of the Gnas gene', Endocrinology, vol. 146, no. 11, pp. 4697-4709. https://doi.org/10.1210/en.2005-0681
Germain-Lee, Emily L. ; Schwindinger, William ; Crane, Janet ; Zewdu, Rediet ; Zweifel, Larry S. ; Wand, Gary S ; Huso, David L. ; Saji, Motoyasu ; Ringel, Matthew D. ; Levine, Michael A. / A mouse model of albright hereditary osteodystrophy generated by targeted disruption of exon 1 of the Gnas gene. In: Endocrinology. 2005 ; Vol. 146, No. 11. pp. 4697-4709.
@article{ce31cc1a9f5749df894ef60815832801,
title = "A mouse model of albright hereditary osteodystrophy generated by targeted disruption of exon 1 of the Gnas gene",
abstract = "Albright hereditary osteodystrophy is caused by heterozygous inactivating mutations in GNAS, a gene that encodes not only the α-chain of Gs (Gαs), but also NESP55 and XLαs through use of alternative first exons. Patients with GNAS mutations on maternally inherited alleles are resistant to multiple hormones such as PTH, TSH, LH/FSH, GHRH, and glucagon, whose receptors are coupled to Gs. This variant of Albright hereditary osteodystrophy is termed pseudohypoparathyroidism type 1a and is due to presumed tissue-specific paternal imprinting of Gαs. Previous studies have shown that mice heterozygous for a targeted disruption of exon 2 of Gnas, the murine homolog of GNAS, showed unique phenotypes dependent on the parent of origin of the mutated allele. However, hormone resistance occurred only when the disrupted gene was maternally inherited. Because disruption of exon 2 is predicted to inactivate Gαs as well as NESP55 and XLαs, we created transgenic mice with disruption of exon 1 to investigate the effects of isolated loss of Gαs. Heterozygous mice that inherited the disruption maternally (-m/+) exhibited PTH and TSH resistance, whereas those with paternal inheritance (+/-p) had normal hormone responsiveness. Heterozygous mice were shorter and, when the disrupted allele was inherited maternally, weighed more than wild-type littermates. Gαs protein and mRNA expression was consistent with paternal imprinting in the renal cortex and thyroid, but there was no imprinting in renal medulla, heart, or adipose. These findings confirm the tissue-specific paternal imprinting of GNAS and demonstrate that Gαs deficiency alone is sufficient to account for the hormone resistance of pseudohypoparathyroidism type 1a.",
author = "Germain-Lee, {Emily L.} and William Schwindinger and Janet Crane and Rediet Zewdu and Zweifel, {Larry S.} and Wand, {Gary S} and Huso, {David L.} and Motoyasu Saji and Ringel, {Matthew D.} and Levine, {Michael A.}",
year = "2005",
month = "11",
doi = "10.1210/en.2005-0681",
language = "English (US)",
volume = "146",
pages = "4697--4709",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "11",

}

TY - JOUR

T1 - A mouse model of albright hereditary osteodystrophy generated by targeted disruption of exon 1 of the Gnas gene

AU - Germain-Lee, Emily L.

AU - Schwindinger, William

AU - Crane, Janet

AU - Zewdu, Rediet

AU - Zweifel, Larry S.

AU - Wand, Gary S

AU - Huso, David L.

AU - Saji, Motoyasu

AU - Ringel, Matthew D.

AU - Levine, Michael A.

PY - 2005/11

Y1 - 2005/11

N2 - Albright hereditary osteodystrophy is caused by heterozygous inactivating mutations in GNAS, a gene that encodes not only the α-chain of Gs (Gαs), but also NESP55 and XLαs through use of alternative first exons. Patients with GNAS mutations on maternally inherited alleles are resistant to multiple hormones such as PTH, TSH, LH/FSH, GHRH, and glucagon, whose receptors are coupled to Gs. This variant of Albright hereditary osteodystrophy is termed pseudohypoparathyroidism type 1a and is due to presumed tissue-specific paternal imprinting of Gαs. Previous studies have shown that mice heterozygous for a targeted disruption of exon 2 of Gnas, the murine homolog of GNAS, showed unique phenotypes dependent on the parent of origin of the mutated allele. However, hormone resistance occurred only when the disrupted gene was maternally inherited. Because disruption of exon 2 is predicted to inactivate Gαs as well as NESP55 and XLαs, we created transgenic mice with disruption of exon 1 to investigate the effects of isolated loss of Gαs. Heterozygous mice that inherited the disruption maternally (-m/+) exhibited PTH and TSH resistance, whereas those with paternal inheritance (+/-p) had normal hormone responsiveness. Heterozygous mice were shorter and, when the disrupted allele was inherited maternally, weighed more than wild-type littermates. Gαs protein and mRNA expression was consistent with paternal imprinting in the renal cortex and thyroid, but there was no imprinting in renal medulla, heart, or adipose. These findings confirm the tissue-specific paternal imprinting of GNAS and demonstrate that Gαs deficiency alone is sufficient to account for the hormone resistance of pseudohypoparathyroidism type 1a.

AB - Albright hereditary osteodystrophy is caused by heterozygous inactivating mutations in GNAS, a gene that encodes not only the α-chain of Gs (Gαs), but also NESP55 and XLαs through use of alternative first exons. Patients with GNAS mutations on maternally inherited alleles are resistant to multiple hormones such as PTH, TSH, LH/FSH, GHRH, and glucagon, whose receptors are coupled to Gs. This variant of Albright hereditary osteodystrophy is termed pseudohypoparathyroidism type 1a and is due to presumed tissue-specific paternal imprinting of Gαs. Previous studies have shown that mice heterozygous for a targeted disruption of exon 2 of Gnas, the murine homolog of GNAS, showed unique phenotypes dependent on the parent of origin of the mutated allele. However, hormone resistance occurred only when the disrupted gene was maternally inherited. Because disruption of exon 2 is predicted to inactivate Gαs as well as NESP55 and XLαs, we created transgenic mice with disruption of exon 1 to investigate the effects of isolated loss of Gαs. Heterozygous mice that inherited the disruption maternally (-m/+) exhibited PTH and TSH resistance, whereas those with paternal inheritance (+/-p) had normal hormone responsiveness. Heterozygous mice were shorter and, when the disrupted allele was inherited maternally, weighed more than wild-type littermates. Gαs protein and mRNA expression was consistent with paternal imprinting in the renal cortex and thyroid, but there was no imprinting in renal medulla, heart, or adipose. These findings confirm the tissue-specific paternal imprinting of GNAS and demonstrate that Gαs deficiency alone is sufficient to account for the hormone resistance of pseudohypoparathyroidism type 1a.

UR - http://www.scopus.com/inward/record.url?scp=26844542000&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26844542000&partnerID=8YFLogxK

U2 - 10.1210/en.2005-0681

DO - 10.1210/en.2005-0681

M3 - Article

VL - 146

SP - 4697

EP - 4709

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 11

ER -