A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells

K. A. O'Connell, M. Edidin

Research output: Contribution to journalArticle

Abstract

Leukocyte-endothelial cell (EC) interactions regulate the entry of immune effectors into the tissues. This interaction occurs in lymphoid tissues and inflammatory sites at post-capillary high endothelial venules, as opposed to large capacitance vessels lined with flat EC. Transient SV40 infection of mouse EC derived from lymph node stroma has resulted in a cell line, SVEC4-10, that retains morphological and functional characteristics of normal EC. SVEC4-10 cells grow efficiently on plastic as a monolayer with a characteristic epithelioid morphology. They require as little as 2% FCS and are independent of other exogenous growth factors or matrix components. When grown on a synthetic basement membrane, SVEC4-10 forms branching tube-like networks. SVEC4-10 expresses Factor VIII related Ag as measured by indirect immunofluorescence using a rabbit antiserum to human FVIII-associated protein and incorporates acetylated low density lipoprotein. SVEC4-10 specifically binds mouse lymphocytes in vitro. IFN-γ induces expression of MHC class II Ag in a time course identical to normal EC and the cell line is susceptible to lysis by anti SV40 H-2(k) CTL clones. Thus, this SV40 immortalized line retains much of the normal cellular physiology of EC.

Original languageEnglish (US)
Pages (from-to)521-525
Number of pages5
JournalJournal of Immunology
Volume144
Issue number2
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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