A monoclonal antibody labels type II neurons of the spiral ganglion

Alice M. Berglund; David K. Ryugo

doi:10.1016/0006-8993(86)90034-X

A monoclonal antibody labels type II neurons of the spiral ganglion

Research output: Contribution to journal › Article › peer-review

Abstract

Tissue from mature rat and cat cochleas was stained with basic dyes in order to distinguish between the cell bodies of type I and II spiral ganglion neurons. This same tissue was then stained with protargol, or processed immunocytochemically using a monoclonal neurofilament antibody, RT-97. Type II neurons were intensely stained, whereas type I neurons were not. We conclude that type II neurons can be clearly distinguished by their abundant neurofilament content.

Original language	English (US)
Pages (from-to)	327-332
Number of pages	6
Journal	Brain research
Volume	383
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(86)90034-X
State	Published - Sep 24 1986
Externally published	Yes

Keywords

Auditory system
Cochlea
Neurofilament
Primary afferent
RT-97
Sensory neuron

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(86)90034-X

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title = "A monoclonal antibody labels type II neurons of the spiral ganglion",

abstract = "Tissue from mature rat and cat cochleas was stained with basic dyes in order to distinguish between the cell bodies of type I and II spiral ganglion neurons. This same tissue was then stained with protargol, or processed immunocytochemically using a monoclonal neurofilament antibody, RT-97. Type II neurons were intensely stained, whereas type I neurons were not. We conclude that type II neurons can be clearly distinguished by their abundant neurofilament content.",

keywords = "Auditory system, Cochlea, Neurofilament, Primary afferent, RT-97, Sensory neuron",

author = "Berglund, {Alice M.} and Ryugo, {David K.}",

note = "Funding Information: manuscript. We are also grateful to P. Ley for photographic assistance, R. Vega for typing assistance, and S. May for technical assistance. This research was supported by NIH Grants NS13126, NS20156, and GM07258. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1986",

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doi = "10.1016/0006-8993(86)90034-X",

language = "English (US)",

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AU - Berglund, Alice M.

AU - Ryugo, David K.

N1 - Funding Information: manuscript. We are also grateful to P. Ley for photographic assistance, R. Vega for typing assistance, and S. May for technical assistance. This research was supported by NIH Grants NS13126, NS20156, and GM07258. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1986/9/24

Y1 - 1986/9/24

N2 - Tissue from mature rat and cat cochleas was stained with basic dyes in order to distinguish between the cell bodies of type I and II spiral ganglion neurons. This same tissue was then stained with protargol, or processed immunocytochemically using a monoclonal neurofilament antibody, RT-97. Type II neurons were intensely stained, whereas type I neurons were not. We conclude that type II neurons can be clearly distinguished by their abundant neurofilament content.

AB - Tissue from mature rat and cat cochleas was stained with basic dyes in order to distinguish between the cell bodies of type I and II spiral ganglion neurons. This same tissue was then stained with protargol, or processed immunocytochemically using a monoclonal neurofilament antibody, RT-97. Type II neurons were intensely stained, whereas type I neurons were not. We conclude that type II neurons can be clearly distinguished by their abundant neurofilament content.

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KW - Cochlea

KW - Neurofilament

KW - Primary afferent

KW - RT-97

KW - Sensory neuron

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