A mono-percoll separation technique improves sperm recovery of normal and male factor specimens when compared with the swim-up technique

S. Shalika, K. Dugan, D. Pelesh, Santiago Padilla

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to determine the effects of a simplified 80% Mono-Percoll sperm separation procedure on both normal and male factor semen samples compared with the standard swim-up technique. The parameters examined include sperm concentration, motility and morphology, total motile functional spermatozoa and percentage recovery. Normal patients demonstrated enhanced sperm parameters with the Mono-Percoll compared with the swim-up technique for concentration (67×106 versus 42×106/ml, P < 0.001), motility (66 versus 59%, P < 0.001), morphology (56 versus 49%, P < 0.005) and percentage recovery (60 versus 42%, P < 0.005). Male factor patients showed enhanced sperm parameters with the Mono-Percoll procedure compared with the swim-up technique for motility (53 versus 42%, P < 0.05) and percentage recovery (54 versus 29%, P < 0.005), with no significant difference in concentration and morphology. In summary, the Mono-Percoll sperm recovery procedure is significantly better than the swim-up technique for male factor patients and patients with normal sperm parameters.

Original languageEnglish (US)
Pages (from-to)3195-3197
Number of pages3
JournalHuman Reproduction
Volume10
Issue number12
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Spermatozoa
Sperm Motility
Semen
Percoll

Keywords

  • Infertility
  • Insemination
  • Mono-Percoll
  • Spermatozoa
  • Swim-up

ASJC Scopus subject areas

  • Reproductive Medicine
  • Rehabilitation
  • Obstetrics and Gynecology

Cite this

A mono-percoll separation technique improves sperm recovery of normal and male factor specimens when compared with the swim-up technique. / Shalika, S.; Dugan, K.; Pelesh, D.; Padilla, Santiago.

In: Human Reproduction, Vol. 10, No. 12, 01.01.1995, p. 3195-3197.

Research output: Contribution to journalArticle

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abstract = "The purpose of this study was to determine the effects of a simplified 80{\%} Mono-Percoll sperm separation procedure on both normal and male factor semen samples compared with the standard swim-up technique. The parameters examined include sperm concentration, motility and morphology, total motile functional spermatozoa and percentage recovery. Normal patients demonstrated enhanced sperm parameters with the Mono-Percoll compared with the swim-up technique for concentration (67×106 versus 42×106/ml, P < 0.001), motility (66 versus 59{\%}, P < 0.001), morphology (56 versus 49{\%}, P < 0.005) and percentage recovery (60 versus 42{\%}, P < 0.005). Male factor patients showed enhanced sperm parameters with the Mono-Percoll procedure compared with the swim-up technique for motility (53 versus 42{\%}, P < 0.05) and percentage recovery (54 versus 29{\%}, P < 0.005), with no significant difference in concentration and morphology. In summary, the Mono-Percoll sperm recovery procedure is significantly better than the swim-up technique for male factor patients and patients with normal sperm parameters.",
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