Abstract
The purpose of this study was to determine the effects of a simplified 80% Mono-Percoll sperm separation procedure on both normal and male factor semen samples compared with the standard swim-up technique. The parameters examined include sperm concentration, motility and morphology, total motile functional spermatozoa and percentage recovery. Normal patients demonstrated enhanced sperm parameters with the Mono-Percoll compared with the swim-up technique for concentration (67×106 versus 42×106/ml, P < 0.001), motility (66 versus 59%, P < 0.001), morphology (56 versus 49%, P < 0.005) and percentage recovery (60 versus 42%, P < 0.005). Male factor patients showed enhanced sperm parameters with the Mono-Percoll procedure compared with the swim-up technique for motility (53 versus 42%, P < 0.05) and percentage recovery (54 versus 29%, P < 0.005), with no significant difference in concentration and morphology. In summary, the Mono-Percoll sperm recovery procedure is significantly better than the swim-up technique for male factor patients and patients with normal sperm parameters.
Original language | English (US) |
---|---|
Pages (from-to) | 3195-3197 |
Number of pages | 3 |
Journal | Human Reproduction |
Volume | 10 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1995 |
Externally published | Yes |
Keywords
- Infertility
- Insemination
- Mono-Percoll
- Spermatozoa
- Swim-up
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology