A molecular switch for the orientation of epithelial cell polarization

David M. Bryant, Julie Roignot, Anirban Datta, Arend W. Overeem, Minji Kim, Wei Yu, Xiao Peng, Dennis J. Eastburn, Andrew Ewald, Zena Werb, Keith E. Mostov

Research output: Contribution to journalArticle

Abstract

The formation of epithelial tissues containing lumens requires not only the apical-basolateral polarization of cells, but also the coordinated orientation of this polarity such that the apical surfaces of neighboring cells all point toward the central lumen. Defects in extracellular matrix (ECM) signaling lead to inverted polarity so that the apical surfaces face the surrounding ECM. We report a molecular switch mechanism controlling polarity orientation. ECM signals through a β1-integrin/FAK/p190RhoGAP complex to downregulate a RhoA/ROCK/Ezrin pathway at the ECM interface. PKCβII phosphorylates the apical identity-promoting Podocalyxin/NHERF1/Ezrin complex, removing Podocalyxin from the ECM-abutting cell surface and initiating its transcytosis to an apical membrane initiation site for lumen formation. Inhibition of this switch mechanism results in the retention of Podocalyxin at the ECM interface and the development instead of collective front-rear polarization and motility. Thus, ECM-derived signals control the morphogenesis of epithelial tissues by controlling the collective orientation of epithelial polarization.

Original languageEnglish (US)
Pages (from-to)171-187
Number of pages17
JournalDevelopmental Cell
Volume31
Issue number2
DOIs
StatePublished - Oct 27 2014

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Extracellular Matrix
Epithelial Cells
Switches
Polarization
Tissue
Integrins
Membranes
Epithelium
Defects
Transcytosis
podocalyxin
Morphogenesis
Down-Regulation
ezrin

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Bryant, D. M., Roignot, J., Datta, A., Overeem, A. W., Kim, M., Yu, W., ... Mostov, K. E. (2014). A molecular switch for the orientation of epithelial cell polarization. Developmental Cell, 31(2), 171-187. https://doi.org/10.1016/j.devcel.2014.08.027

A molecular switch for the orientation of epithelial cell polarization. / Bryant, David M.; Roignot, Julie; Datta, Anirban; Overeem, Arend W.; Kim, Minji; Yu, Wei; Peng, Xiao; Eastburn, Dennis J.; Ewald, Andrew; Werb, Zena; Mostov, Keith E.

In: Developmental Cell, Vol. 31, No. 2, 27.10.2014, p. 171-187.

Research output: Contribution to journalArticle

Bryant, DM, Roignot, J, Datta, A, Overeem, AW, Kim, M, Yu, W, Peng, X, Eastburn, DJ, Ewald, A, Werb, Z & Mostov, KE 2014, 'A molecular switch for the orientation of epithelial cell polarization', Developmental Cell, vol. 31, no. 2, pp. 171-187. https://doi.org/10.1016/j.devcel.2014.08.027
Bryant DM, Roignot J, Datta A, Overeem AW, Kim M, Yu W et al. A molecular switch for the orientation of epithelial cell polarization. Developmental Cell. 2014 Oct 27;31(2):171-187. https://doi.org/10.1016/j.devcel.2014.08.027
Bryant, David M. ; Roignot, Julie ; Datta, Anirban ; Overeem, Arend W. ; Kim, Minji ; Yu, Wei ; Peng, Xiao ; Eastburn, Dennis J. ; Ewald, Andrew ; Werb, Zena ; Mostov, Keith E. / A molecular switch for the orientation of epithelial cell polarization. In: Developmental Cell. 2014 ; Vol. 31, No. 2. pp. 171-187.
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