A molecular scheme for improved characterization of human embryonic stem cell lines

Richard Josephson, Gregory Sykes, Ying Liu, Carol Ording, Weining Xu, Xianmin Zeng, Soojung Shin, Jeanne Loring, Anirban Maitra, Mahendra S. Rao, Jonathan M. Auerbach

Research output: Contribution to journalArticle

Abstract

Background: Human embryonic stem cells (hESC) offer a renewable source of a wide range of cell types for use in research and cell-based therapies to treat disease. Inspection of protein markers provides important information about the current state of the cells and data for subsequent manipulations. However, hESC must be routinely analyzed at the genomic level to guard against deleterious changes during extensive propagation, expansion, and manipulation in vitro. Results: We found that short tandem repeat (STR) analysis, human leukocyte antigen (HLA) typing, single nucleotide polymorphism (SNP) genomic analysis, mitochondrial DNA sequencing, and gene expression analysis by microarray can be used to fully describe any hESC culture in terms of its identity, stability, and undifferentiated state. Conclusion: Here we describe, using molecular biology alone, a comprehensive characterization of 17 different hESC lines. The use of amplified nucleic acids means that for the first time full characterization of hESC lines can be performed with little time investment and a minimum of material. The information thus gained will facilitate comparison of lines and replication of results between laboratories.

Original languageEnglish (US)
Article number28
JournalBMC Biology
Volume4
DOIs
StatePublished - Aug 18 2006

ASJC Scopus subject areas

  • Biotechnology
  • Structural Biology
  • Developmental Biology
  • Physiology
  • Plant Science
  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

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  • Cite this

    Josephson, R., Sykes, G., Liu, Y., Ording, C., Xu, W., Zeng, X., Shin, S., Loring, J., Maitra, A., Rao, M. S., & Auerbach, J. M. (2006). A molecular scheme for improved characterization of human embryonic stem cell lines. BMC Biology, 4, [28]. https://doi.org/10.1186/1741-7007-4-28