TY - JOUR
T1 - A molecular scheme for improved characterization of human embryonic stem cell lines
AU - Josephson, Richard
AU - Sykes, Gregory
AU - Liu, Ying
AU - Ording, Carol
AU - Xu, Weining
AU - Zeng, Xianmin
AU - Shin, Soojung
AU - Loring, Jeanne
AU - Maitra, Anirban
AU - Rao, Mahendra S.
AU - Auerbach, Jonathan M.
PY - 2006/8/18
Y1 - 2006/8/18
N2 - Background: Human embryonic stem cells (hESC) offer a renewable source of a wide range of cell types for use in research and cell-based therapies to treat disease. Inspection of protein markers provides important information about the current state of the cells and data for subsequent manipulations. However, hESC must be routinely analyzed at the genomic level to guard against deleterious changes during extensive propagation, expansion, and manipulation in vitro. Results: We found that short tandem repeat (STR) analysis, human leukocyte antigen (HLA) typing, single nucleotide polymorphism (SNP) genomic analysis, mitochondrial DNA sequencing, and gene expression analysis by microarray can be used to fully describe any hESC culture in terms of its identity, stability, and undifferentiated state. Conclusion: Here we describe, using molecular biology alone, a comprehensive characterization of 17 different hESC lines. The use of amplified nucleic acids means that for the first time full characterization of hESC lines can be performed with little time investment and a minimum of material. The information thus gained will facilitate comparison of lines and replication of results between laboratories.
AB - Background: Human embryonic stem cells (hESC) offer a renewable source of a wide range of cell types for use in research and cell-based therapies to treat disease. Inspection of protein markers provides important information about the current state of the cells and data for subsequent manipulations. However, hESC must be routinely analyzed at the genomic level to guard against deleterious changes during extensive propagation, expansion, and manipulation in vitro. Results: We found that short tandem repeat (STR) analysis, human leukocyte antigen (HLA) typing, single nucleotide polymorphism (SNP) genomic analysis, mitochondrial DNA sequencing, and gene expression analysis by microarray can be used to fully describe any hESC culture in terms of its identity, stability, and undifferentiated state. Conclusion: Here we describe, using molecular biology alone, a comprehensive characterization of 17 different hESC lines. The use of amplified nucleic acids means that for the first time full characterization of hESC lines can be performed with little time investment and a minimum of material. The information thus gained will facilitate comparison of lines and replication of results between laboratories.
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U2 - 10.1186/1741-7007-4-28
DO - 10.1186/1741-7007-4-28
M3 - Article
C2 - 16919167
AN - SCOPUS:33750097804
SN - 1741-7007
VL - 4
JO - BMC Biology
JF - BMC Biology
M1 - 28
ER -