A modified adjusting delay task to assess impulsive choice between isocaloric reinforcers in non-deprived male rats: Effects of 5-HT 2A/C and 5-HT 1A receptor agonists

Angelo Blasio, Aditi R. Narayan, Barbara J. Kaminski, Luca Steardo, Valentina Sabino, Pietro Cottone

Research output: Contribution to journalArticle

Abstract

Rationale: Existing animal models of impulsivity frequently use food restriction to increase subjects' motivation. In addition, behavioral tasks that assess impulsive choice typically involve the use of reinforcers with dissimilar caloric content. These factors represent energy-homeostasis limitations, which may confound the interpretation of results and limit the applicability of these models. Objectives: This study was aimed at validating face and convergent validities of a modified adjusting delay task, which assesses impulsive choice between isocaloric reinforcers in ad libitum fed rats. Methods: Male Wistar rats (n∈=∈18) were used to assess the preferredness and reinforcing efficacy of a "supersaccharin" solution (1.5% glucose/0.4% saccharin) over a 1.5% glucose solution. A separate group of rats (n∈=∈24) was trained in a modified adjusting delay task, which involved repeated choice between the glucose solution delivered immediately and the supersaccharin solution delivered after a variable delay. To pharmacologically validate the task, the effects of the 5-HT 2A/C receptor agonist (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane [(±)-DOI] and the 5-HT 1A receptor agonist (±)-8- hydroxy-2-(dipropylamino)tetralin hydrobromide [(±)-8-OH-DPAT] on impulsive choice were then evaluated. Results: Supersaccharin was highly reinforcing and uniformly preferred over the glucose solution by all subjects. Rats quickly learned the task, and impulsivity was a very stable and consistent trait. DOI and 8-OH-DPAT significantly and dose dependently increased impulsive choice in this modified adjusting delay task. Conclusions: We validated a rodent task of impulsive choice, which eliminates typical energy-homeostasis limitations and, therefore, opens new avenues in the study of impulsivity in preclinical feeding and obesity research.

Original languageEnglish (US)
Pages (from-to)377-386
Number of pages10
JournalPsychopharmacology
Volume219
Issue number2
DOIs
StatePublished - Jan 2012

Fingerprint

Receptor, Serotonin, 5-HT1A
Serotonin
Impulsive Behavior
Glucose
8-Hydroxy-2-(di-n-propylamino)tetralin
Homeostasis
Receptor, Serotonin, 5-HT2A
Saccharin
Reproducibility of Results
Wistar Rats
Motivation
Rodentia
Animal Models
Obesity
Food
Research

Keywords

  • Decision making
  • Delay discounting
  • Food deprivation
  • Food restriction
  • Impulsivity
  • Serotonin

ASJC Scopus subject areas

  • Pharmacology

Cite this

A modified adjusting delay task to assess impulsive choice between isocaloric reinforcers in non-deprived male rats : Effects of 5-HT 2A/C and 5-HT 1A receptor agonists. / Blasio, Angelo; Narayan, Aditi R.; Kaminski, Barbara J.; Steardo, Luca; Sabino, Valentina; Cottone, Pietro.

In: Psychopharmacology, Vol. 219, No. 2, 01.2012, p. 377-386.

Research output: Contribution to journalArticle

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abstract = "Rationale: Existing animal models of impulsivity frequently use food restriction to increase subjects' motivation. In addition, behavioral tasks that assess impulsive choice typically involve the use of reinforcers with dissimilar caloric content. These factors represent energy-homeostasis limitations, which may confound the interpretation of results and limit the applicability of these models. Objectives: This study was aimed at validating face and convergent validities of a modified adjusting delay task, which assesses impulsive choice between isocaloric reinforcers in ad libitum fed rats. Methods: Male Wistar rats (n∈=∈18) were used to assess the preferredness and reinforcing efficacy of a {"}supersaccharin{"} solution (1.5{\%} glucose/0.4{\%} saccharin) over a 1.5{\%} glucose solution. A separate group of rats (n∈=∈24) was trained in a modified adjusting delay task, which involved repeated choice between the glucose solution delivered immediately and the supersaccharin solution delivered after a variable delay. To pharmacologically validate the task, the effects of the 5-HT 2A/C receptor agonist (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane [(±)-DOI] and the 5-HT 1A receptor agonist (±)-8- hydroxy-2-(dipropylamino)tetralin hydrobromide [(±)-8-OH-DPAT] on impulsive choice were then evaluated. Results: Supersaccharin was highly reinforcing and uniformly preferred over the glucose solution by all subjects. Rats quickly learned the task, and impulsivity was a very stable and consistent trait. DOI and 8-OH-DPAT significantly and dose dependently increased impulsive choice in this modified adjusting delay task. Conclusions: We validated a rodent task of impulsive choice, which eliminates typical energy-homeostasis limitations and, therefore, opens new avenues in the study of impulsivity in preclinical feeding and obesity research.",
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AU - Narayan, Aditi R.

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AU - Cottone, Pietro

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