A model of human immunodeficiency virus encephalitis in scid mice

William R. Tyor, Christopher Power, Howard E. Gendelman, Richard Bryan Markham

Research output: Contribution to journalArticle

Abstract

Human immunodeficiency virus (HIV)-associated dementia complex is a common and devastating manifestation of the late phases of HIV infection. The pathogenesis of dementia complex is poorly understood and effective treatments have not been developed, in part because of the lack of an appropriate animal model. Mice with severe combined immunodeficiency (scid mice), which accept xenografts without rejection, were intracerebrally inoculated with human peripheral blood mononuclear cells and HIV. One to 4 weeks after inoculation, the brains of these mice contained human macrophages (some of which were HIV p24 antigen positive), occasional multinucleated cells, and striking gliosis by immunocytochemical staining. Human macrophages also were frequently positive for tumor necrosis factor type a and occasionally for interleukin 1 and VLA-4. Cultures of these brains for HIV were positive. Generally, human macrophages were not present in the brains of control mice, nor was significant gliosis, and HIV was not recovered from mice that received HIV only intracerebrally. Pathologically, this model of HIV encephalitis in scid mice resembles HIV encephalitis in humans and the data suggest that the activation of macrophages by infection with HIV results in their accumulation and persistence in brain and in the development of gliosis. This model of HIV encephalitis should provide insights into the pathogenesis and treatment of this disorder.

Original languageEnglish (US)
Pages (from-to)8658-8662
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number18
StatePublished - Sep 15 1993

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Severe Combined Immunodeficiency
Encephalitis
HIV
Gliosis
Macrophages
Brain
Dementia
Integrin alpha4beta1
Macrophage Activation
Virus Diseases
Interleukin-1
Heterografts
Blood Cells
Animal Models
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A model of human immunodeficiency virus encephalitis in scid mice. / Tyor, William R.; Power, Christopher; Gendelman, Howard E.; Markham, Richard Bryan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 18, 15.09.1993, p. 8658-8662.

Research output: Contribution to journalArticle

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