A model for transgenerational imprinting variation in complex traits

Chenguang Wang, Zhong Wang, Jiangtao Luo, Qin Li, Yao Li, Kwangmi Ahn, Daniel R. Prows, Rongling Wu

Research output: Contribution to journalArticle

Abstract

Despite the fact that genetic imprinting, i.e., differential expression of the same allele due to its different parental origins, plays a pivotal role in controlling complex traits or diseases, the origin, action and transmission mode of imprinted genes have still remained largely unexplored. We present a new strategy for studying these properties of genetic imprinting with a two-stage reciprocal F2 mating design, initiated with two contrasting inbred lines. This strategy maps quantitative trait loci that are imprinted (i.e., iQTLs) based on their segregation and transmission across different generations. By incorporating the allelic configuration of an iQTL genotype into a mixture model framework, this strategy provides a path to trace the parental origin of alleles from previous generations. The imprinting effects of iQTLs and their interactions with other traditionally defined genetic effects, expressed in different generations, are estimated and tested by implementing the EM algorithm. The strategy was used to map iQTLs responsible for survival time with four reciprocal F2 populations and test whether and how the detected iQTLs inherit their imprinting effects into the next generation. The new strategy will provide a tool for quantifying the role of imprinting effects in the creation and maintenance of phenotypic diversity and elucidating a comprehensive picture of the genetic architecture of complex traits and diseases.

Original languageEnglish (US)
Article numbere11396
JournalPLoS One
Volume5
Issue number7
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Genomic Imprinting
genomic imprinting
Alleles
Quantitative Trait Loci
Genes
Genotype
Maintenance
genotype mixtures
alleles
Population
inbred lines
quantitative trait loci
genes
testing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A model for transgenerational imprinting variation in complex traits. / Wang, Chenguang; Wang, Zhong; Luo, Jiangtao; Li, Qin; Li, Yao; Ahn, Kwangmi; Prows, Daniel R.; Wu, Rongling.

In: PLoS One, Vol. 5, No. 7, e11396, 2010.

Research output: Contribution to journalArticle

Wang, C, Wang, Z, Luo, J, Li, Q, Li, Y, Ahn, K, Prows, DR & Wu, R 2010, 'A model for transgenerational imprinting variation in complex traits', PLoS One, vol. 5, no. 7, e11396. https://doi.org/10.1371/journal.pone.0011396
Wang, Chenguang ; Wang, Zhong ; Luo, Jiangtao ; Li, Qin ; Li, Yao ; Ahn, Kwangmi ; Prows, Daniel R. ; Wu, Rongling. / A model for transgenerational imprinting variation in complex traits. In: PLoS One. 2010 ; Vol. 5, No. 7.
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