A model for the prediction of relative titres of avian malaria and aspergillus spp. IgG in jackass penguin (Spheniscus demersus) females based on maternal IgG in egg-yolk

Thaddeus K. Graczyk, Michael R. Cranfield

Research output: Contribution to journalArticle

Abstract

A model for the prediction of IgG titres in females of the Jackass penguin (Spheniscus demersus) was developed, based on IgG which was maternally transmitted to the yolk of unembryonated eggs produced by these females. However, prediction of the female titre based on the titre of embryonated eggs may be inadequate. Brood samples from 10 S. demersus females and their corresponding embryonated (n = 10) and unembryonated (n = 49) eggs were analysed by indirect ELISA for avian malaria (Plasmodium relictum, P. elongatum) IgG and Aspergillus spp. IgG. There was no correlation between humoral responses to avian malaria and Aspergillus spp. in females or in their eggs. Avian malaria and Aspergillus spp. titres were significantly higher (P <0.05) in the eggs than in the corresponding females and were significantly correlated (P <0.01) with the blood titres, r = 0.84, r = 0.89, respectively. No correlation was found between titres of embryo yolk-sac (embryonated eggs) and the blood of their female parent; however, the embryo blood and the corresponding female titres were significantly (P <0.05) correlated (avian malaria, r = 0.74; Aspergillus spp., r = 0.69). Blood and the corresponding egg-yolk (unembryonated eggs) lgG titres regressed significantly (P <0.01). Female IgG titre (y)) is related to (unembryonated) egg-yolk IgG titre by the significant (P <0.05) regression y = - 0.61 + 1.46X for avian malaria, and y = -0.02 + 0.85X for Aspergillus spp., with ±95% prediction limits of ±0.15 and ±0.12, respectively. This model provides access to serological information on the remote free-ranging Jackass penguins and captive Jackass-penguin colonies without the necessity of stressful blood collection.

Original languageEnglish (US)
Pages (from-to)749-754
Number of pages6
JournalInternational Journal for Parasitology
Volume26
Issue number7
DOIs
StatePublished - Jul 1996

Fingerprint

Avian Malaria
Spheniscidae
Egg Yolk
Aspergillus
Immunoglobulin G
Mothers
Eggs
Embryonic Structures
Access to Information
Yolk Sac
Plasmodium
Enzyme-Linked Immunosorbent Assay

Keywords

  • African black-footed penguins
  • Aspergillosis
  • Aspergillus spp
  • Avian malaria
  • ELISA
  • Jackass penguins
  • Maternal IgG
  • Spheniscus demersus

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

A model for the prediction of relative titres of avian malaria and aspergillus spp. IgG in jackass penguin (Spheniscus demersus) females based on maternal IgG in egg-yolk. / Graczyk, Thaddeus K.; Cranfield, Michael R.

In: International Journal for Parasitology, Vol. 26, No. 7, 07.1996, p. 749-754.

Research output: Contribution to journalArticle

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abstract = "A model for the prediction of IgG titres in females of the Jackass penguin (Spheniscus demersus) was developed, based on IgG which was maternally transmitted to the yolk of unembryonated eggs produced by these females. However, prediction of the female titre based on the titre of embryonated eggs may be inadequate. Brood samples from 10 S. demersus females and their corresponding embryonated (n = 10) and unembryonated (n = 49) eggs were analysed by indirect ELISA for avian malaria (Plasmodium relictum, P. elongatum) IgG and Aspergillus spp. IgG. There was no correlation between humoral responses to avian malaria and Aspergillus spp. in females or in their eggs. Avian malaria and Aspergillus spp. titres were significantly higher (P <0.05) in the eggs than in the corresponding females and were significantly correlated (P <0.01) with the blood titres, r = 0.84, r = 0.89, respectively. No correlation was found between titres of embryo yolk-sac (embryonated eggs) and the blood of their female parent; however, the embryo blood and the corresponding female titres were significantly (P <0.05) correlated (avian malaria, r = 0.74; Aspergillus spp., r = 0.69). Blood and the corresponding egg-yolk (unembryonated eggs) lgG titres regressed significantly (P <0.01). Female IgG titre (y)) is related to (unembryonated) egg-yolk IgG titre by the significant (P <0.05) regression y = - 0.61 + 1.46X for avian malaria, and y = -0.02 + 0.85X for Aspergillus spp., with ±95{\%} prediction limits of ±0.15 and ±0.12, respectively. This model provides access to serological information on the remote free-ranging Jackass penguins and captive Jackass-penguin colonies without the necessity of stressful blood collection.",
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