A model for generating paired complete blood count and oblique back-illumination capillaroscopy data in tissue-realistic microfluidic chambers

Gregory N. McKay, Luojie Huang, Taylor L. Bobrow, Srivathsan Kalyan, Soojung Claire Hur, Sophie Lanzkron, Lydia H. Pecker, Alison R. Moliterno, Nicholas J. Durr

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Oblique back-illumination capillaroscopy (OBC) has recently demonstrated clear images of unlabeled human blood cells in vivo. Combined with deep learning-based algorithms, this technology may enable non-invasive blood cell counting and analysis as flowing red blood cells, platelets, and white blood cells can be observed in their native environment. To harness the full potential of OBC, new techniques and methods must be developed that provide ground truth data using human blood cells. Here we present such a model, where human blood cells with paired ground truth information are imaged flowing in a custom tissue-mimicking micro fluidic device. This model enables the acquisition of OBC datasets that will help with both training and validating machine learning models for applications including the complete blood count, specific blood cell classification, and the study of hematologic disorders such as anemia.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX
EditorsAttila Tarnok, Jessica P. Houston
PublisherSPIE
ISBN (Electronic)9781510647992
DOIs
StatePublished - 2022
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX 2022 - Virtual, Online
Duration: Feb 20 2022Feb 24 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11964
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX 2022
CityVirtual, Online
Period2/20/222/24/22

Keywords

  • blood cell imaging
  • Capillaroscopy
  • hematology
  • microfluidics
  • oblique back-illumination

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'A model for generating paired complete blood count and oblique back-illumination capillaroscopy data in tissue-realistic microfluidic chambers'. Together they form a unique fingerprint.

Cite this