Abstract
Mass correlated acceleration (MCA) has now been integrated into a 4 in (10.2 cm) matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer to achieve high resolving power across a broader mass range, without sacrificing detection of higher mass ions. The goal was to combine MCA with a custom-built miniaturized instrument such as those that might be used for field-portable applications. Unlike other pulsed extraction methods, MCA is not mass dependent and mass spectra can be achieved with a single tuning of instrument parameters. Additionally, the multi-channel recording advantage is better realized because ions of all masses can be brought into focus simultaneously. The MCA dual-stage ion source compensates dynamically for the mass dependence by incorporating an extraction pulse region followed by an acceleration region that contains a time-dependent waveform correlated with mass. The technique was validated with applications in peptide mixtures and protein digestions. Diagnostic studies for the instrument include m/z range and limits of detection.
Original language | English (US) |
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Pages (from-to) | 296-304 |
Number of pages | 9 |
Journal | Journal of Mass Spectrometry |
Volume | 38 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1 2003 |
Keywords
- Field application
- Mass-correlated acceleration focusing
- Miniaturized matrix-assisted laser desorption/ionization time-of-flight mass spectrometer
- Peptides
- Proteins
ASJC Scopus subject areas
- Spectroscopy