A miniaturized matrix-assisted laser desorption/ionization time of flight mass spectrometer with mass-correlated acceleration focusing

Robert D. English, Robert J. Cotter

Research output: Contribution to journalArticlepeer-review

Abstract

Mass correlated acceleration (MCA) has now been integrated into a 4 in (10.2 cm) matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer to achieve high resolving power across a broader mass range, without sacrificing detection of higher mass ions. The goal was to combine MCA with a custom-built miniaturized instrument such as those that might be used for field-portable applications. Unlike other pulsed extraction methods, MCA is not mass dependent and mass spectra can be achieved with a single tuning of instrument parameters. Additionally, the multi-channel recording advantage is better realized because ions of all masses can be brought into focus simultaneously. The MCA dual-stage ion source compensates dynamically for the mass dependence by incorporating an extraction pulse region followed by an acceleration region that contains a time-dependent waveform correlated with mass. The technique was validated with applications in peptide mixtures and protein digestions. Diagnostic studies for the instrument include m/z range and limits of detection.

Original languageEnglish (US)
Pages (from-to)296-304
Number of pages9
JournalJournal of Mass Spectrometry
Volume38
Issue number3
DOIs
StatePublished - Mar 1 2003

Keywords

  • Field application
  • Mass-correlated acceleration focusing
  • Miniaturized matrix-assisted laser desorption/ionization time-of-flight mass spectrometer
  • Peptides
  • Proteins

ASJC Scopus subject areas

  • Spectroscopy

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