A micromethod for the purification of lysyl oxidase

Rocco Ferrera; Barbara Faris; Peter J. Mogayzel; Wayne A. Gonnerman; Carl Franzblau

doi:10.1016/0003-2697(82)90521-8

A micromethod for the purification of lysyl oxidase

Rocco Ferrera, Barbara Faris, Peter J. Mogayzel, Wayne A. Gonnerman, Carl Franzblau

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A simple, efficient, and rapid procedure for the purification of lysyl oxidase is described. This method utilizes an affinity scheme involving powdered elastin-hydroxyethylmethacrylate hydrogels and high-performance liquid chromatography and permits the study of enzyme from sources which contain limited amounts of enzyme, such as aortic smooth muscle cells in culture.

Original language	English (US)
Pages (from-to)	312-317
Number of pages	6
Journal	Analytical biochemistry
Volume	126
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(82)90521-8
State	Published - Nov 1 1982
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(82)90521-8

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title = "A micromethod for the purification of lysyl oxidase",

abstract = "A simple, efficient, and rapid procedure for the purification of lysyl oxidase is described. This method utilizes an affinity scheme involving powdered elastin-hydroxyethylmethacrylate hydrogels and high-performance liquid chromatography and permits the study of enzyme from sources which contain limited amounts of enzyme, such as aortic smooth muscle cells in culture.",

author = "Rocco Ferrera and Barbara Faris and Mogayzel, {Peter J.} and Gonnerman, {Wayne A.} and Carl Franzblau",

note = "Funding Information: The authors gratefully acknowledge the expert technical assistance of Ms. V. Verbitzki and Ms. R. Mos-caritolo. This study was supported by NIH Grants HL19717 and HL13262.",

year = "1982",

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doi = "10.1016/0003-2697(82)90521-8",

language = "English (US)",

volume = "126",

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T1 - A micromethod for the purification of lysyl oxidase

AU - Ferrera, Rocco

AU - Faris, Barbara

AU - Mogayzel, Peter J.

AU - Gonnerman, Wayne A.

AU - Franzblau, Carl

N1 - Funding Information: The authors gratefully acknowledge the expert technical assistance of Ms. V. Verbitzki and Ms. R. Mos-caritolo. This study was supported by NIH Grants HL19717 and HL13262.

PY - 1982/11/1

Y1 - 1982/11/1

N2 - A simple, efficient, and rapid procedure for the purification of lysyl oxidase is described. This method utilizes an affinity scheme involving powdered elastin-hydroxyethylmethacrylate hydrogels and high-performance liquid chromatography and permits the study of enzyme from sources which contain limited amounts of enzyme, such as aortic smooth muscle cells in culture.

AB - A simple, efficient, and rapid procedure for the purification of lysyl oxidase is described. This method utilizes an affinity scheme involving powdered elastin-hydroxyethylmethacrylate hydrogels and high-performance liquid chromatography and permits the study of enzyme from sources which contain limited amounts of enzyme, such as aortic smooth muscle cells in culture.

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U2 - 10.1016/0003-2697(82)90521-8

DO - 10.1016/0003-2697(82)90521-8

M3 - Article

C2 - 6130722

AN - SCOPUS:0020446428

SN - 0003-2697

VL - 126

SP - 312

EP - 317

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

A micromethod for the purification of lysyl oxidase

Abstract

ASJC Scopus subject areas

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