A microfluidic device with groove patterns for studying cellular behavior

Bong Geun Chung, Amir Manbachi, Ali Khademhosseini

Research output: Contribution to journalArticle

Abstract

We describe a microfluidic device with microgrooved patterns for studying cellular behavior. This microfluidic platform consists of a top fluidic channel and a bottom microgrooved substrate. To fabricate the microgrooved channels, a top poly(dimethylsiloxane) (PDMS) mold containing the impression of the microfluidic channels was aligned and bonded to a microgrooved substrate. Using this device, mouse fibroblast cells were immobilized and patterned within microgrooved substrates (25, 50, 75, and 100 μm wide). To study apoptosis in a microfluidic device, media containing hydrogen peroxide, Annexin V, and propidium iodide was perfused into the fluidic channel for 2 hours. We found that cells exposed to the oxidative stress became apoptotic. These apoptotic cells were confirmed by Annexin V that bound to phosphatidylserine at the outer leaflet of the plasma membrane during the apoptosis process. Using this microfluidic device with microgrooved patterns, the apoptosis process was observed in real-time and analyzed by using an inverted microscope containing an incubation chamber (37°C, 5% CO2). Therefore, this microfluidic device incorporated with microgrooved substrates could be useful for studying the cellular behavior and performing high-throughput drug screening.

Original languageEnglish (US)
Article numbere270
JournalJournal of Visualized Experiments
Issue number7
DOIs
StatePublished - Aug 2007
Externally publishedYes

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
Annexin A5
Cell death
Apoptosis
Fluidics
Substrates
Immobilized Cells
Preclinical Drug Evaluations
Propidium
Phosphatidylserines
Cells
Hydrogen Peroxide
Oxidative Stress
Fungi
Oxidative stress
Fibroblasts
Cell Membrane
Polydimethylsiloxane
Cell membranes

Keywords

  • Apoptosis
  • Cell biology
  • Issue 7
  • Microfluidic
  • Tissue engineering

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A microfluidic device with groove patterns for studying cellular behavior. / Chung, Bong Geun; Manbachi, Amir; Khademhosseini, Ali.

In: Journal of Visualized Experiments, No. 7, e270, 08.2007.

Research output: Contribution to journalArticle

@article{0a4664326cae4683933b4f9d0b8ab0ea,
title = "A microfluidic device with groove patterns for studying cellular behavior",
abstract = "We describe a microfluidic device with microgrooved patterns for studying cellular behavior. This microfluidic platform consists of a top fluidic channel and a bottom microgrooved substrate. To fabricate the microgrooved channels, a top poly(dimethylsiloxane) (PDMS) mold containing the impression of the microfluidic channels was aligned and bonded to a microgrooved substrate. Using this device, mouse fibroblast cells were immobilized and patterned within microgrooved substrates (25, 50, 75, and 100 μm wide). To study apoptosis in a microfluidic device, media containing hydrogen peroxide, Annexin V, and propidium iodide was perfused into the fluidic channel for 2 hours. We found that cells exposed to the oxidative stress became apoptotic. These apoptotic cells were confirmed by Annexin V that bound to phosphatidylserine at the outer leaflet of the plasma membrane during the apoptosis process. Using this microfluidic device with microgrooved patterns, the apoptosis process was observed in real-time and analyzed by using an inverted microscope containing an incubation chamber (37°C, 5{\%} CO2). Therefore, this microfluidic device incorporated with microgrooved substrates could be useful for studying the cellular behavior and performing high-throughput drug screening.",
keywords = "Apoptosis, Cell biology, Issue 7, Microfluidic, Tissue engineering",
author = "Chung, {Bong Geun} and Amir Manbachi and Ali Khademhosseini",
year = "2007",
month = "8",
doi = "10.3791/270",
language = "English (US)",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "7",

}

TY - JOUR

T1 - A microfluidic device with groove patterns for studying cellular behavior

AU - Chung, Bong Geun

AU - Manbachi, Amir

AU - Khademhosseini, Ali

PY - 2007/8

Y1 - 2007/8

N2 - We describe a microfluidic device with microgrooved patterns for studying cellular behavior. This microfluidic platform consists of a top fluidic channel and a bottom microgrooved substrate. To fabricate the microgrooved channels, a top poly(dimethylsiloxane) (PDMS) mold containing the impression of the microfluidic channels was aligned and bonded to a microgrooved substrate. Using this device, mouse fibroblast cells were immobilized and patterned within microgrooved substrates (25, 50, 75, and 100 μm wide). To study apoptosis in a microfluidic device, media containing hydrogen peroxide, Annexin V, and propidium iodide was perfused into the fluidic channel for 2 hours. We found that cells exposed to the oxidative stress became apoptotic. These apoptotic cells were confirmed by Annexin V that bound to phosphatidylserine at the outer leaflet of the plasma membrane during the apoptosis process. Using this microfluidic device with microgrooved patterns, the apoptosis process was observed in real-time and analyzed by using an inverted microscope containing an incubation chamber (37°C, 5% CO2). Therefore, this microfluidic device incorporated with microgrooved substrates could be useful for studying the cellular behavior and performing high-throughput drug screening.

AB - We describe a microfluidic device with microgrooved patterns for studying cellular behavior. This microfluidic platform consists of a top fluidic channel and a bottom microgrooved substrate. To fabricate the microgrooved channels, a top poly(dimethylsiloxane) (PDMS) mold containing the impression of the microfluidic channels was aligned and bonded to a microgrooved substrate. Using this device, mouse fibroblast cells were immobilized and patterned within microgrooved substrates (25, 50, 75, and 100 μm wide). To study apoptosis in a microfluidic device, media containing hydrogen peroxide, Annexin V, and propidium iodide was perfused into the fluidic channel for 2 hours. We found that cells exposed to the oxidative stress became apoptotic. These apoptotic cells were confirmed by Annexin V that bound to phosphatidylserine at the outer leaflet of the plasma membrane during the apoptosis process. Using this microfluidic device with microgrooved patterns, the apoptosis process was observed in real-time and analyzed by using an inverted microscope containing an incubation chamber (37°C, 5% CO2). Therefore, this microfluidic device incorporated with microgrooved substrates could be useful for studying the cellular behavior and performing high-throughput drug screening.

KW - Apoptosis

KW - Cell biology

KW - Issue 7

KW - Microfluidic

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=80355126437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80355126437&partnerID=8YFLogxK

U2 - 10.3791/270

DO - 10.3791/270

M3 - Article

C2 - 18989441

AN - SCOPUS:80355126437

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 7

M1 - e270

ER -