A microfluidic device for kinetic optimization of protein crystallization and in situ structure determination

Carl L. Hansen, Scott Classen, James M. Berger, Stephen R. Quake

Research output: Contribution to journalArticle

Abstract

The unprecedented economies of scale and unique mass transport properties of microfluidic devices made them viable nano-volume protein crystallization screening platforms. However, realizing the full potential of microfluidic crystallization requires complementary technologies for crystal optimization and harvesting. In this paper, we report a microfluidic device which provides a link between chip-based nanoliter volume crystallization screening and structure analysis through "kinetic optimization" of crystallization reactions and in situ structure determination. Kinetic optimization through systematic variation of reactor geometry and actuation of micromechanical valves is used to screen a large ensemble of kinetic trajectories that are not practical with conventional techniques. Using this device, we demonstrate control over crystal quality, reliable scale-up from nanoliter volume reactions, facile harvesting and cryoprotectant screening, and protein structure determination at atomic resolution from data collected in-chip.

Original languageEnglish (US)
Pages (from-to)3142-3143
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number10
DOIs
StatePublished - Mar 15 2006
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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