A microarray-based genetic screen for yeast chronological aging factors

Mirela Matecic, Daniel L. Smith, Xuewen Pan, Nazif Maqani, Stefan Bekiranov, Jef D. Boeke, Jeffrey S. Smith

Research output: Contribution to journalArticle

Abstract

Model organisms have played an important role in the elucidation of multiple genes and cellular processes that regulate aging. In this study we utilized the budding yeast, Saccharomyces cerevisiae, in a large-scale screen for genes that function in the regulation of chronological lifespan, which is defined by the number of days that non-dividing cells remain viable. A pooled collection of viable haploid gene deletion mutants, each tagged with unique identifying DNA ''bar-code'' sequences was chronologically aged in liquid culture. Viable mutants in the aging population were selected at several time points and then detected using a microarray DNA hybridization technique that quantifies abundance of the barcode tags. Multiple short- and long-lived mutants were identified using this approach. Among the confirmed short-lived mutants were those defective for autophagy, indicating a key requirement for the recycling of cellular organelles in longevity. Defects in autophagy also prevented lifespan extension induced by limitation of amino acids in the growth media. Among the confirmed long-lived mutants were those defective in the highly conserved de novo purine biosynthesis pathway (the ADE genes), which ultimately produces IMP and AMP. Blocking this pathway extended lifespan to the same degree as calorie (glucose) restriction. A recently discovered cell-extrinsic mechanism of chronological aging involving acetic acid secretion and toxicity was suppressed in a long-lived ade4D mutant and exacerbated by a short-lived atg16D autophagy mutant. The identification of multiple novel effectors of yeast chronological lifespan will greatly aid in the elucidation of mechanisms that cells and organisms utilize in slowing down the aging process.

Original languageEnglish (US)
JournalPLoS Genetics
Volume6
Issue number4
DOIs
StatePublished - Apr 2010

Fingerprint

microbial genetics
Autophagy
yeast
Yeasts
mutants
autophagy
Genes
Inosine Monophosphate
Saccharomycetales
Haploidy
Gene Deletion
Adenosine Monophosphate
Oligonucleotide Array Sequence Analysis
Automatic Data Processing
gene
barcoding
Acetic Acid
Organelles
Saccharomyces cerevisiae
Amino Acids

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Matecic, M., Smith, D. L., Pan, X., Maqani, N., Bekiranov, S., Boeke, J. D., & Smith, J. S. (2010). A microarray-based genetic screen for yeast chronological aging factors. PLoS Genetics, 6(4). https://doi.org/10.1371/journal.pgen.1000921

A microarray-based genetic screen for yeast chronological aging factors. / Matecic, Mirela; Smith, Daniel L.; Pan, Xuewen; Maqani, Nazif; Bekiranov, Stefan; Boeke, Jef D.; Smith, Jeffrey S.

In: PLoS Genetics, Vol. 6, No. 4, 04.2010.

Research output: Contribution to journalArticle

Matecic, M, Smith, DL, Pan, X, Maqani, N, Bekiranov, S, Boeke, JD & Smith, JS 2010, 'A microarray-based genetic screen for yeast chronological aging factors', PLoS Genetics, vol. 6, no. 4. https://doi.org/10.1371/journal.pgen.1000921
Matecic M, Smith DL, Pan X, Maqani N, Bekiranov S, Boeke JD et al. A microarray-based genetic screen for yeast chronological aging factors. PLoS Genetics. 2010 Apr;6(4). https://doi.org/10.1371/journal.pgen.1000921
Matecic, Mirela ; Smith, Daniel L. ; Pan, Xuewen ; Maqani, Nazif ; Bekiranov, Stefan ; Boeke, Jef D. ; Smith, Jeffrey S. / A microarray-based genetic screen for yeast chronological aging factors. In: PLoS Genetics. 2010 ; Vol. 6, No. 4.
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