A method for transforming human liver epithelial cells by transfection using a plasmid containing SV40 early region gene

T. Tokiwa, M. M. Lipsky, D. T. Smoot, J. F. Lechner

Research output: Contribution to journalArticlepeer-review

Abstract

A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes.

Original languageEnglish (US)
Pages (from-to)57-60
Number of pages4
JournalJournal of Tissue Culture Methods
Volume16
Issue number1
DOIs
StatePublished - Mar 1 1994

Keywords

  • Human hepatocytes
  • SV40 T antigen gene
  • Transfection
  • Transformed epithelial cell line

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

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