Abstract
A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes.
Original language | English (US) |
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Pages (from-to) | 57-60 |
Number of pages | 4 |
Journal | Journal of Tissue Culture Methods |
Volume | 16 |
Issue number | 1 |
DOIs | |
State | Published - Mar 1994 |
Externally published | Yes |
Keywords
- Human hepatocytes
- SV40 T antigen gene
- Transfection
- Transformed epithelial cell line
ASJC Scopus subject areas
- Anatomy
- Pathology and Forensic Medicine