A method for transforming human liver epithelial cells by transfection using a plasmid containing SV40 early region gene

T. Tokiwa; M. M. Lipsky; D. T. Smoot; J. F. Lechner

doi:10.1007/BF01404836

A method for transforming human liver epithelial cells by transfection using a plasmid containing SV40 early region gene

T. Tokiwa, M. M. Lipsky, D. T. Smoot, J. F. Lechner

Research output: Contribution to journal › Article › peer-review

Abstract

A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×10⁵ hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes.

Original language	English (US)
Pages (from-to)	57-60
Number of pages	4
Journal	Journal of Tissue Culture Methods
Volume	16
Issue number	1
DOIs	https://doi.org/10.1007/BF01404836
State	Published - Mar 1994
Externally published	Yes

Keywords

Human hepatocytes
SV40 T antigen gene
Transfection
Transformed epithelial cell line

ASJC Scopus subject areas

Anatomy
Pathology and Forensic Medicine

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10.1007/BF01404836

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abstract = "A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes.",

keywords = "Human hepatocytes, SV40 T antigen gene, Transfection, Transformed epithelial cell line",

author = "T. Tokiwa and Lipsky, {M. M.} and Smoot, {D. T.} and Lechner, {J. F.}",

year = "1994",

month = mar,

doi = "10.1007/BF01404836",

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pages = "57--60",

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AU - Tokiwa, T.

AU - Lipsky, M. M.

AU - Smoot, D. T.

AU - Lechner, J. F.

PY - 1994/3

Y1 - 1994/3

N2 - A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes.

AB - A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes.

KW - Human hepatocytes

KW - SV40 T antigen gene

KW - Transfection

KW - Transformed epithelial cell line

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A method for transforming human liver epithelial cells by transfection using a plasmid containing SV40 early region gene

Abstract

Keywords

ASJC Scopus subject areas

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