A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes.
- Human hepatocytes
- SV40 T antigen gene
- Transformed epithelial cell line
ASJC Scopus subject areas
- Pathology and Forensic Medicine