Abstract
A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different nonradioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 441-448 |
| Number of pages | 8 |
| Journal | Leprosy Review |
| Volume | 72 |
| Issue number | 4 |
| State | Published - 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Dermatology
Cite this
A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae. / Honoré, Nadine; Roche, Paul W.; Grosset, Jacques H.; Cole, Stewart T.
In: Leprosy Review, Vol. 72, No. 4, 2001, p. 441-448.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae
AU - Honoré, Nadine
AU - Roche, Paul W.
AU - Grosset, Jacques H.
AU - Cole, Stewart T.
PY - 2001
Y1 - 2001
N2 - A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different nonradioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
AB - A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different nonradioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035701150&partnerID=8YFLogxK
M3 - Article
C2 - 11826480
AN - SCOPUS:0035701150
VL - 72
SP - 441
EP - 448
JO - Leprosy Review
JF - Leprosy Review
SN - 0305-7518
IS - 4
ER -