A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae

Nadine Honoré; Paul W. Roche; Jacques H. Grosset; Stewart T. Cole

doi:10.5935/0305-7518.20010052

A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae

Nadine Honoré, Paul W. Roche, Jacques H. Grosset, Stewart T. Cole

School of Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different nonradioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.

Original language	English (US)
Pages (from-to)	441-448
Number of pages	8
Journal	Leprosy review
Volume	72
Issue number	4
DOIs	https://doi.org/10.5935/0305-7518.20010052
State	Published - 2001

ASJC Scopus subject areas

Dermatology
Infectious Diseases

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abstract = "A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different nonradioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.",

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AB - A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different nonradioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.

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A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae

Abstract

ASJC Scopus subject areas

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