A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae

Nadine Honoré, Paul W. Roche, Jacques H. Grosset, Stewart T. Cole

Research output: Contribution to journalArticle

Abstract

A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different nonradioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalLeprosy review
Volume72
Issue number4
DOIs
StatePublished - Jan 1 2001

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ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

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