A method for hepatic arterial perfusion studies in the rat

Thuy T. Than, James V. Sitzmann, Shao Sen Li, Pin Wen Lin, Louise B. Grochow

Research output: Contribution to journalArticle

Abstract

The current model for selective hepatic arterial infusion therapy was evaluated for its suitability for short-term pharmacokinetic experiments. This preparation consists of selective cannulation of the common hepatic artery via the gastroduodenal artery of the rat. Radioactive microspheres were injected to determine hepatic or extrahepatic sites of perfusion. Radioactive microsphere determination of hepatic arterial flow and cardiac output were also performed. Our data indicate that at high flow rates (2 ml/min), significant loss of drug would occur due to retrograde flow. Modification of the model to include temporary proximal hepatic artery occlusion ensures hepatic delivery of greater than 95% of drug. Furthermore, temporary hepatic artery occlusion does not alter cardiac output or hepatic artery occlusion does not alter cardiac output or hepatic arterial blood flow. Selective hepatic arterial infusion in rats with synchronous temporary hepatic artery occlusion is an adequate model for short-term pharmacokinetic studies.

Original languageEnglish (US)
Pages (from-to)251-254
Number of pages4
JournalJournal of Surgical Research
Volume47
Issue number3
DOIs
StatePublished - Sep 1989

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ASJC Scopus subject areas

  • Surgery

Cite this

Than, T. T., Sitzmann, J. V., Li, S. S., Lin, P. W., & Grochow, L. B. (1989). A method for hepatic arterial perfusion studies in the rat. Journal of Surgical Research, 47(3), 251-254. https://doi.org/10.1016/0022-4804(89)90116-9