A method for fixation of previously fresh-frozen human adult and fetal brains that preserves histological quality and immunoreactivity

E. G. Jones; S. H.C. Hendry; X. B. Liu; S. Hodgins; S. G. Potkin; W. W. Tourtellotte

doi:10.1016/0165-0270(92)90006-Y

A method for fixation of previously fresh-frozen human adult and fetal brains that preserves histological quality and immunoreactivity

E. G. Jones, S. H.C. Hendry, X. B. Liu, S. Hodgins, S. G. Potkin, W. W. Tourtellotte

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

A method is described that enables fixation of previously fresh-frozen and stored adult and fetal human or animal brains. The method involves fixing during thawing under controlled, cryoprotected conditions and is compatible with good histological quality and the preservation of enzymatic activity and immunoreactivity of many neural antigens. It offers considerable advantages for the storage of large amounts of tissue from which multiple samples can be taken and processed under fixation and other conditions that can be optimized for a variety of methods, many of which may be incompatible if the whole brain is fixed in a single fixative prior to storage.

Original language	English (US)
Pages (from-to)	133-144
Number of pages	12
Journal	Journal of Neuroscience Methods
Volume	44
Issue number	2-3
DOIs	https://doi.org/10.1016/0165-0270(92)90006-Y
State	Published - Sep 1992
Externally published	Yes

Keywords

Brain storage
Cryoprotection
Histochemistry
Immunocytochemistry
Thaw fixation

ASJC Scopus subject areas

General Neuroscience

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10.1016/0165-0270(92)90006-Y

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title = "A method for fixation of previously fresh-frozen human adult and fetal brains that preserves histological quality and immunoreactivity",

abstract = "A method is described that enables fixation of previously fresh-frozen and stored adult and fetal human or animal brains. The method involves fixing during thawing under controlled, cryoprotected conditions and is compatible with good histological quality and the preservation of enzymatic activity and immunoreactivity of many neural antigens. It offers considerable advantages for the storage of large amounts of tissue from which multiple samples can be taken and processed under fixation and other conditions that can be optimized for a variety of methods, many of which may be incompatible if the whole brain is fixed in a single fixative prior to storage.",

keywords = "Brain storage, Cryoprotection, Histochemistry, Immunocytochemistry, Thaw fixation",

author = "Jones, {E. G.} and Hendry, {S. H.C.} and Liu, {X. B.} and S. Hodgins and Potkin, {S. G.} and Tourtellotte, {W. W.}",

note = "Funding Information: Supported by Grant No. MH44188 from the National Institute of Mental Health, United States Public Health Service. We thank Tony Vilhena, Phong Nguyen, Linda Lam and Hao Truong for technical help and Dr. S.A. Mednick, Dr. S. Leveillee and Mr. Thor Mednick for supplying the fetal brains. Adult brains were obtained using the facilities of the National Neurological Research Specimen Bank, VAMC Wads-",

year = "1992",

month = sep,

doi = "10.1016/0165-0270(92)90006-Y",

language = "English (US)",

volume = "44",

pages = "133--144",

journal = "Journal of Neuroscience Methods",

issn = "0165-0270",

publisher = "Elsevier",

number = "2-3",

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TY - JOUR

T1 - A method for fixation of previously fresh-frozen human adult and fetal brains that preserves histological quality and immunoreactivity

AU - Jones, E. G.

AU - Hendry, S. H.C.

AU - Liu, X. B.

AU - Hodgins, S.

AU - Potkin, S. G.

AU - Tourtellotte, W. W.

N1 - Funding Information: Supported by Grant No. MH44188 from the National Institute of Mental Health, United States Public Health Service. We thank Tony Vilhena, Phong Nguyen, Linda Lam and Hao Truong for technical help and Dr. S.A. Mednick, Dr. S. Leveillee and Mr. Thor Mednick for supplying the fetal brains. Adult brains were obtained using the facilities of the National Neurological Research Specimen Bank, VAMC Wads-

PY - 1992/9

Y1 - 1992/9

N2 - A method is described that enables fixation of previously fresh-frozen and stored adult and fetal human or animal brains. The method involves fixing during thawing under controlled, cryoprotected conditions and is compatible with good histological quality and the preservation of enzymatic activity and immunoreactivity of many neural antigens. It offers considerable advantages for the storage of large amounts of tissue from which multiple samples can be taken and processed under fixation and other conditions that can be optimized for a variety of methods, many of which may be incompatible if the whole brain is fixed in a single fixative prior to storage.

AB - A method is described that enables fixation of previously fresh-frozen and stored adult and fetal human or animal brains. The method involves fixing during thawing under controlled, cryoprotected conditions and is compatible with good histological quality and the preservation of enzymatic activity and immunoreactivity of many neural antigens. It offers considerable advantages for the storage of large amounts of tissue from which multiple samples can be taken and processed under fixation and other conditions that can be optimized for a variety of methods, many of which may be incompatible if the whole brain is fixed in a single fixative prior to storage.

KW - Brain storage

KW - Cryoprotection

KW - Histochemistry

KW - Immunocytochemistry

KW - Thaw fixation

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SN - 0165-0270

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JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

IS - 2-3

ER -

A method for fixation of previously fresh-frozen human adult and fetal brains that preserves histological quality and immunoreactivity

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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