Abstract
When the inhalation anesthetic halothane was administered to rats, a 58 kDa protein in the liver became covalently labeled by the trifluoroacetyl chloride metabolite of halothane. The amino acid sequences of the N-terminal and of several internal peptide fragments of the protein were 99% homologous to that of the deduced amino acid sequence of a cDNA reported to correspond to phosphatidylinositol-specific phospholipase C-α. The purified trifluoroacetylated 58 kDa protein or native 58 kDa protein, however, did not have phosphatidylinositol-specific phospholipase C activity. We conclude that the reported cDNA of phosphatidylinositol-specific phospholipase C-α may encode for a microsomal protein of unknown function.
Original language | English (US) |
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Pages (from-to) | 679-685 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 178 |
Issue number | 2 |
DOIs | |
State | Published - Jul 31 1991 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology