A member of a conserved Plasmodium protein family with membrane-attack complex/perforin (MACPF)-like domains localizes to the micronemes of sporozoites

Karine Kaiser, Nelly Camargo, Isabelle Coppens, Joanne M. Morrisey, Akhil B. Vaidya, Stefan H I Kappe

Research output: Contribution to journalArticle

Abstract

Pore-forming proteins are employed by many pathogens to achieve successful host colonization. Intracellular pathogens use pore-forming proteins to invade host cells, survive within and productively interact with host cells, and finally egress from host cells to infect new ones. The malaria-causing parasites of the genus Plasmodium evolved a number of life cycle stages that enter and replicate in distinct cell types within the mosquito vector and vertebrate host. Despite the fact that interaction with host-cell membranes is a central theme in the Plasmodium life cycle, little is known about parasite proteins that mediate such interactions. We identified a family of five related genes in the genome of the rodent malaria parasite Plasmodium yoelii encoding secreted proteins all bearing a single membrane-attack complex/perforin (MACPF)-like domain. Each protein is highly conserved among Plasmodium species. Gene expression analysis in P. yoelii and the human malaria parasite Plasmodium falciparum indicated that the family is not expressed in the parasites blood stages. However, one of the genes was significantly expressed in P. yoelii sporozoites, the stage transmitted by mosquito bite. The protein localized to the micronemes of sporozoites, organelles of the secretory invasion apparatus intimately involved in host-cell infection. MACPF-like proteins may play important roles in parasite interactions with the mosquito vector and transmission to the vertebrate host.

Original languageEnglish (US)
Pages (from-to)15-26
Number of pages12
JournalMolecular and Biochemical Parasitology
Volume133
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Complement Membrane Attack Complex
Sporozoites
Perforin
Plasmodium
Parasites
Plasmodium yoelii
Porins
Proteins
Life Cycle Stages
Malaria
Vertebrates
Falciparum Malaria
Bites and Stings
Culicidae
Organelles
Genes
Rodentia
Cell Membrane
Genome
Gene Expression

Keywords

  • Gene expression
  • Host-cell invasion
  • Malaria
  • Multigene family
  • Plasmodium yoelii
  • Pore-forming protein
  • Stage-specific expression

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

A member of a conserved Plasmodium protein family with membrane-attack complex/perforin (MACPF)-like domains localizes to the micronemes of sporozoites. / Kaiser, Karine; Camargo, Nelly; Coppens, Isabelle; Morrisey, Joanne M.; Vaidya, Akhil B.; Kappe, Stefan H I.

In: Molecular and Biochemical Parasitology, Vol. 133, No. 1, 01.2004, p. 15-26.

Research output: Contribution to journalArticle

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