A manganese-rich environment supports superoxide dismutase activity in a lyme disease pathogen, borrelia burgdorferi

J. Dafhne Aguirre, Hillary M. Clark, Matthew McIlvin, Christine Vazquez, Shaina L. Palmere, Dennis J. Grab, J. Seshu, P. John Hart, Mak Saito, Valeria L Culotta

Research output: Contribution to journalArticle

Abstract

The Lyme disease pathogen Borrelia burgdorferi represents a novel organism in which to study metalloprotein biology in that this spirochete has uniquely evolved with no requirement for iron. Not only is iron low, but we show here that B. burgdorferi has the capacity to accumulate remarkably high levels of manganese. This high manganese is necessary to activate the SodA superoxide dismutase (SOD) essential for virulence. Using a metalloproteomic approach, we demonstrate that a bulk of B. burgdorferi SodA directly associates with manganese, and a smaller pool of inactive enzyme accumulates as apoprotein. Other metalloproteins may have similarly adapted to using manganese as co-factor, including the BB0366 aminopeptidase. Whereas B. burgdorferi SodA has evolved in a manganese-rich, iron-poor environment, the opposite is true for Mn-SODs of organisms such as Escherichia coli and bakers' yeast. These Mn- SODs still capture manganese in an iron-rich cell, and we tested whether the same is true for Borrelia SodA. When expressed in the iron-rich mitochondria of Saccharomyces cerevisiae, B. burgdorferi SodA was inactive. Activity was only possible when cells accumulated extremely high levels of manganese that exceeded cellular iron. Moreover, there was no evidence for iron inactivation of the SOD. B. burgdorferi SodA shows strong overall homology with other members of the Mn-SOD family, but computer-assisted modeling revealed some unusual features of the hydrogen bonding network near the enzyme's active site. The unique properties of B. burgdorferi SodA may represent adaptation to expression in the manganese-rich and iron-poor environment of the spirochete.

Original languageEnglish (US)
Pages (from-to)8468-8478
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number12
DOIs
StatePublished - Mar 22 2013

Fingerprint

Borrelia burgdorferi
Lyme Disease
Pathogens
Manganese
Superoxide Dismutase
Iron
Metalloproteins
Spirochaetales
Yeast
Saccharomyces cerevisiae
Borrelia
Aminopeptidases
Mitochondria
Apoproteins
Enzymes
Hydrogen Bonding
Escherichia coli
Virulence
Catalytic Domain
Hydrogen bonds

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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A manganese-rich environment supports superoxide dismutase activity in a lyme disease pathogen, borrelia burgdorferi. / Aguirre, J. Dafhne; Clark, Hillary M.; McIlvin, Matthew; Vazquez, Christine; Palmere, Shaina L.; Grab, Dennis J.; Seshu, J.; Hart, P. John; Saito, Mak; Culotta, Valeria L.

In: Journal of Biological Chemistry, Vol. 288, No. 12, 22.03.2013, p. 8468-8478.

Research output: Contribution to journalArticle

Aguirre, JD, Clark, HM, McIlvin, M, Vazquez, C, Palmere, SL, Grab, DJ, Seshu, J, Hart, PJ, Saito, M & Culotta, VL 2013, 'A manganese-rich environment supports superoxide dismutase activity in a lyme disease pathogen, borrelia burgdorferi', Journal of Biological Chemistry, vol. 288, no. 12, pp. 8468-8478. https://doi.org/10.1074/jbc.M112.433540
Aguirre, J. Dafhne ; Clark, Hillary M. ; McIlvin, Matthew ; Vazquez, Christine ; Palmere, Shaina L. ; Grab, Dennis J. ; Seshu, J. ; Hart, P. John ; Saito, Mak ; Culotta, Valeria L. / A manganese-rich environment supports superoxide dismutase activity in a lyme disease pathogen, borrelia burgdorferi. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 12. pp. 8468-8478.
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AU - Clark, Hillary M.

AU - McIlvin, Matthew

AU - Vazquez, Christine

AU - Palmere, Shaina L.

AU - Grab, Dennis J.

AU - Seshu, J.

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AU - Culotta, Valeria L

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N2 - The Lyme disease pathogen Borrelia burgdorferi represents a novel organism in which to study metalloprotein biology in that this spirochete has uniquely evolved with no requirement for iron. Not only is iron low, but we show here that B. burgdorferi has the capacity to accumulate remarkably high levels of manganese. This high manganese is necessary to activate the SodA superoxide dismutase (SOD) essential for virulence. Using a metalloproteomic approach, we demonstrate that a bulk of B. burgdorferi SodA directly associates with manganese, and a smaller pool of inactive enzyme accumulates as apoprotein. Other metalloproteins may have similarly adapted to using manganese as co-factor, including the BB0366 aminopeptidase. Whereas B. burgdorferi SodA has evolved in a manganese-rich, iron-poor environment, the opposite is true for Mn-SODs of organisms such as Escherichia coli and bakers' yeast. These Mn- SODs still capture manganese in an iron-rich cell, and we tested whether the same is true for Borrelia SodA. When expressed in the iron-rich mitochondria of Saccharomyces cerevisiae, B. burgdorferi SodA was inactive. Activity was only possible when cells accumulated extremely high levels of manganese that exceeded cellular iron. Moreover, there was no evidence for iron inactivation of the SOD. B. burgdorferi SodA shows strong overall homology with other members of the Mn-SOD family, but computer-assisted modeling revealed some unusual features of the hydrogen bonding network near the enzyme's active site. The unique properties of B. burgdorferi SodA may represent adaptation to expression in the manganese-rich and iron-poor environment of the spirochete.

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