A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia

Michael B. Kastan, Qimin Zhan, Wafik S. El-Deiry, France Carrier, Tyler Jacks, William V. Walsh, Beverly S. Plunkett, Bert Vogelstein, Albert J. Fornace

Research output: Contribution to journalArticle

Abstract

Cell cycle checkpoints can enhance cell survival and limit mutagenic events following DNA damage. Primary murine fibroblasts became deficient in a G1 checkpoint activated by ionizing radiation (IR) when both wild-type p53 alleles were disrupted. In addition, cells from patients with the radiosensitive, cancer-prone disease ataxia-telangiectasia (AT) lacked the IR-induced increase in p53 protein levels seen in normal cells. Finally, IR induction of the human GADD45 gene, an induction that is also defective in AT cells, was dependent on wild-type p53 function. Wild-type but not mutant p53 bound strongly to a conserved element in the GADD45 gene, and a p53-containing nuclear factor, which bound this element, was detected in extracts from irradiated cells. Thus, we identified three participants (AT gene(s), p53, and GADD45) in a signal transduction pathway that controls cell cycle arrest following DNA damage; abnormalities in this pathway probably contribute to tumor development.

Original languageEnglish (US)
Pages (from-to)587-597
Number of pages11
JournalCell
Volume71
Issue number4
DOIs
StatePublished - Nov 13 1992

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Ataxia Telangiectasia
Cell Cycle Checkpoints
Ionizing Radiation
Ionizing radiation
p53 Genes
Cells
DNA Damage
Genes
Cell Extracts
Signal transduction
Signal Transduction
DNA
Neoplasms
Cell Survival
Fibroblasts
Alleles
Tumors
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Kastan, M. B., Zhan, Q., El-Deiry, W. S., Carrier, F., Jacks, T., Walsh, W. V., ... Fornace, A. J. (1992). A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. Cell, 71(4), 587-597. https://doi.org/10.1016/0092-8674(92)90593-2

A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. / Kastan, Michael B.; Zhan, Qimin; El-Deiry, Wafik S.; Carrier, France; Jacks, Tyler; Walsh, William V.; Plunkett, Beverly S.; Vogelstein, Bert; Fornace, Albert J.

In: Cell, Vol. 71, No. 4, 13.11.1992, p. 587-597.

Research output: Contribution to journalArticle

Kastan, MB, Zhan, Q, El-Deiry, WS, Carrier, F, Jacks, T, Walsh, WV, Plunkett, BS, Vogelstein, B & Fornace, AJ 1992, 'A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia', Cell, vol. 71, no. 4, pp. 587-597. https://doi.org/10.1016/0092-8674(92)90593-2
Kastan, Michael B. ; Zhan, Qimin ; El-Deiry, Wafik S. ; Carrier, France ; Jacks, Tyler ; Walsh, William V. ; Plunkett, Beverly S. ; Vogelstein, Bert ; Fornace, Albert J. / A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. In: Cell. 1992 ; Vol. 71, No. 4. pp. 587-597.
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