A lymphokine that activates the cytolytic program of both cytotoxic T lymphocyte and natural killer clones

C. Milanese, Robert F Siliciano, R. E. Schmidt, J. Ritz, N. E. Richardson, E. L. Reinherz

Research output: Contribution to journalArticle

Abstract

A 10-12 kD lymphokine, herein termed TCAF, was recently shown to be secreted from Th after crosslinking of their antigen/MHC (T3-Ti) receptors. TCAF stimulates resting T lymphocyte proliferation via binding to surface components of the T11 pathway. To determine whether TCAF could induce antigen-independent activation of the lytic machinery of cytotoxic cells, the present studies were conducted. In the presence of TCAF, both T8+ class I MHC-specific and T4+ class II MHC-specific cytotoxic T cell clones were induced to kill targets, including those lacking the appropriate MHC molecules. This effect was unique to TCAF, since IL-1, IL-2, IFN-γ could not stimulate lytic activity. Furthermore, both T3+T11+ and T3-T11+ NK clones were triggered to lyse NK-resistant target cells. These findings suggest that TCAF can function in an antigen-independent fashion to amplify cytotoxic effector responses.

Original languageEnglish (US)
Pages (from-to)1583-1588
Number of pages6
JournalJournal of Experimental Medicine
Volume163
Issue number6
StatePublished - 1986
Externally publishedYes

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Lymphokines
Cytotoxic T-Lymphocytes
Clone Cells
Antigens
T-Lymphocytes
Thyroid Hormone Receptors
Interleukin-1
Interleukin-2

ASJC Scopus subject areas

  • Immunology

Cite this

Milanese, C., Siliciano, R. F., Schmidt, R. E., Ritz, J., Richardson, N. E., & Reinherz, E. L. (1986). A lymphokine that activates the cytolytic program of both cytotoxic T lymphocyte and natural killer clones. Journal of Experimental Medicine, 163(6), 1583-1588.

A lymphokine that activates the cytolytic program of both cytotoxic T lymphocyte and natural killer clones. / Milanese, C.; Siliciano, Robert F; Schmidt, R. E.; Ritz, J.; Richardson, N. E.; Reinherz, E. L.

In: Journal of Experimental Medicine, Vol. 163, No. 6, 1986, p. 1583-1588.

Research output: Contribution to journalArticle

Milanese, C, Siliciano, RF, Schmidt, RE, Ritz, J, Richardson, NE & Reinherz, EL 1986, 'A lymphokine that activates the cytolytic program of both cytotoxic T lymphocyte and natural killer clones', Journal of Experimental Medicine, vol. 163, no. 6, pp. 1583-1588.
Milanese, C. ; Siliciano, Robert F ; Schmidt, R. E. ; Ritz, J. ; Richardson, N. E. ; Reinherz, E. L. / A lymphokine that activates the cytolytic program of both cytotoxic T lymphocyte and natural killer clones. In: Journal of Experimental Medicine. 1986 ; Vol. 163, No. 6. pp. 1583-1588.
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AU - Reinherz, E. L.

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