A live human parainfluenza type 3 virus vaccine is attenuated and immunogenic in healthy infants and children

Ruth A. Karron, Peter F. Wright, Frances K. Newman, Mamodikoe Makhene, Juliette Thompson, Roberta Samorodin, Modena H. Wilson, Edwin L. Anderson, Mary Lou Clements, Brian R. Murphy, Robert B. Belshe

Research output: Contribution to journalArticle

Abstract

The safety, infectivity, immunogenicity, and phenotypic stability of the cold-passaged (cp) candidate vaccine cp-45, a cold-adapted (ca), temperature-sensitive (ts) mutant of the J8 strain of human parainfluenza virus type 3 (HPIV-3), was evaluated in 114 children 6 months to 10 years old in a randomized, placebo-controlled, double-blind trial. The cp-45 vaccine was well tolerated when given intranasally to parainfluenza virus type 3 (PIV-3)-seropositive and -seronegative children. With 104or 105TCID50of cp-45 vaccine, 86% of seronegative vaccinees were infected, 83% of whom shed virus at a mean peak titer of 102.2pfu/mL. Virus present in respiratory specimens retained the ts phenotype, and each of 86 PIV-3 isolates tested retained both the ca and ts phenotypes. One dose of 105TCID50of vaccine induced a serum hemagglutination-inhibiting antibody response in 81% of vaccinees; the geometric mean titer was 1:32. These studies indicate that the cp-45 HPIV-3 vaccine is satisfactorily attenuated, infectious, immunogenic, and phenotypically stable and merits further evaluation in infants and young children.

Original languageEnglish (US)
Number of pages1
JournalJournal of Infectious Diseases
Volume172
Issue number6
DOIs
StatePublished - Dec 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Karron, R. A., Wright, P. F., Newman, F. K., Makhene, M., Thompson, J., Samorodin, R., Wilson, M. H., Anderson, E. L., Clements, M. L., Murphy, B. R., & Belshe, R. B. (1995). A live human parainfluenza type 3 virus vaccine is attenuated and immunogenic in healthy infants and children. Journal of Infectious Diseases, 172(6). https://doi.org/10.1093/infdis/172.6.1445