A limited region within hen egg-white lysozyme serves as the focus for a diversity of T cell clones

F. Manca, J. A. Clarke, A. Miller, E. E. Sercarz, Nilabh Shastri

Research output: Contribution to journalArticle

Abstract

The C57BL/6 (H-2(b)) mouse is a nonresponder to hen egg-white lysozyme (HEL) injected i.p., owing to a T suppressor cell-inducing determinant at the amino-terminal region. After immunization with a 93-amino acid fragment (a.a. 13-105) of HEL lacking this determinant, all clones from two independently derived C57BL/6 T cell lines were found to be specific for epitopes within a subregion of peptide 74-96. Three specificity patterns for the clones could be defined on the basis of cross-reactivities with only two other species variant lysozymes. Reactivities of all three specificity groups were consistent with the serine to threonine substitution at position 91, although reactivity of one of the groups could be affected by substitutions at position 84. The results confirm at the clonal level that even for distantly related antigens, only limited regions are recognized by T cells. They are consistent with the notion that specific sites on the antigen capable of interaction with Ia molecules lead to dominance of certain regions for T cell reactivity. Moreover, the diversity in specificity among clones suggests that the limiting features of T cell responsiveness is not a lack of available T cells in the repertoire directed against a single antigenic site.

Original languageEnglish (US)
Pages (from-to)2075-2078
Number of pages4
JournalJournal of Immunology
Volume133
Issue number4
StatePublished - Nov 8 1984
Externally publishedYes

Fingerprint

Egg White
Clone Cells
T-Lymphocytes
Antigens
Threonine
Muramidase
Serine
Epitopes
Immunization
hen egg lysozyme
Amino Acids
Cell Line

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

A limited region within hen egg-white lysozyme serves as the focus for a diversity of T cell clones. / Manca, F.; Clarke, J. A.; Miller, A.; Sercarz, E. E.; Shastri, Nilabh.

In: Journal of Immunology, Vol. 133, No. 4, 08.11.1984, p. 2075-2078.

Research output: Contribution to journalArticle

Manca, F. ; Clarke, J. A. ; Miller, A. ; Sercarz, E. E. ; Shastri, Nilabh. / A limited region within hen egg-white lysozyme serves as the focus for a diversity of T cell clones. In: Journal of Immunology. 1984 ; Vol. 133, No. 4. pp. 2075-2078.
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