A large scale method of separating multiple lymphokines secreted by the murine el-4 thymoma

Alfred E. Chang, Michael T. Lotze, Robert S. Ames, Jack A. Roth, Steven A. Rosenberg

Research output: Contribution to journalArticlepeer-review

Abstract

The in vivo administration of lymphokines is a new approach to immuno-therapy with possible applications to the treatment of tumors and infectious processes. These lymphokines are produced in very small quantities and purification of these molecules has proven difficult. A rapid two step method was used to isolate different lymphokines from large quantities of conditioned media produced by the murine EL-4 cell line. Interleukin-2 (IL-2), colony stimulating factor (CSF) and nacrophage activating factor (MAF) are lymphokines with distinct biologic activities and are secreted by the EL-4 cell line. Their isolation involved initial batch purification on trimthylsilyl-controlled pore glass beads followed by reversed phase high pressure liquid chromatography. Recovery of IL-2 activity was >100% of initial activity. The specific activity of the purified IL-2 ranged from 2×106 to 3.6×107 U/mg protein. Colony stimulating factor (CSF) and macrophage activating factor (MAF) were also separated on the chromatographic columns. This technique is useful for the purification of large quantities of these lymphokines which have potential in vivo applications.

Original languageEnglish (US)
Pages (from-to)17-31
Number of pages15
JournalImmunopharmacology and Immunotoxicology
Volume7
Issue number1
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Toxicology
  • Pharmacology

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