A lamin-independent pathway for nuclear envelope assembly

John W. Newport, Katherine Lee Wilson, William G. Dunphy

Research output: Contribution to journalArticle

Abstract

The nuclear envelope is composed of membranes, nuclear pores, and a nuclear lamina. Using a cell-free nuclear assembly extract derived from Xenopus eggs, we have investigated how these three components interact during nuclear assembly. We find that the Xenopus embryonic lamin protein LIII cannot bind directly to chromatin or membranes when each is present alone, but is readily incorporated into nuclei when both of the components are present together in an assembly extract. We find that depleting lamin LIII from an extract does not prevent formation of an envelope consisting of membranes and nuclear pores. However, these lamin-depleted envelopes are extremely fragile and fail to grow beyond a limited extent. This suggests that lamin assembly is not required during the initial steps of nuclear envelope formation, but is required for later growth and for maintaining the structural integrity of the envelope. We also present results showing that lamins may only be incorporated into nuclei after DNA has been encapsulated within an envelope and nuclear transport has been activated. With respect to nuclear function, our results show that the presence of a nuclear lamina is required for DNA synthesis to occur within assembled nuclei.

Original languageEnglish (US)
Pages (from-to)2247-2259
Number of pages13
JournalJournal of Cell Biology
Volume111
Issue number6 PART 1
StatePublished - Dec 1990
Externally publishedYes

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Lamins
Nuclear Envelope
Nuclear Lamina
Nuclear Pore
Xenopus
Membranes
Cell Nucleus Active Transport
DNA
Eggs
Chromatin
Growth
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Newport, J. W., Wilson, K. L., & Dunphy, W. G. (1990). A lamin-independent pathway for nuclear envelope assembly. Journal of Cell Biology, 111(6 PART 1), 2247-2259.

A lamin-independent pathway for nuclear envelope assembly. / Newport, John W.; Wilson, Katherine Lee; Dunphy, William G.

In: Journal of Cell Biology, Vol. 111, No. 6 PART 1, 12.1990, p. 2247-2259.

Research output: Contribution to journalArticle

Newport, JW, Wilson, KL & Dunphy, WG 1990, 'A lamin-independent pathway for nuclear envelope assembly', Journal of Cell Biology, vol. 111, no. 6 PART 1, pp. 2247-2259.
Newport, John W. ; Wilson, Katherine Lee ; Dunphy, William G. / A lamin-independent pathway for nuclear envelope assembly. In: Journal of Cell Biology. 1990 ; Vol. 111, No. 6 PART 1. pp. 2247-2259.
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