A knotted free minicircle in kinetoplast DNA.

K. A. Ryan, T. A. Shapiro, C. A. Rauch, J. D. Griffith, P. T. Englund

Research output: Contribution to journalArticlepeer-review

Abstract

Kinetoplast DNA, the mitochondrial DNA of trypanosomes, is a network containing thousands of minicircles that are topologically interlocked. The minicircle replication intermediates are free molecules that have been released from the network. We report here that one form of free minicircles is a trefoil knot. Identification of this knotted structure is based on its electrophoretic and sedimentation properties, its response to treatments with restriction enzymes or topoisomerase II, and its appearance by electron microscopy. Except for its topology, the knotted minicircle closely resembles a previously described replication intermediate with a unique gap in the newly synthesized L strand.

Original languageEnglish (US)
Pages (from-to)5844-5848
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number16
DOIs
StatePublished - Aug 1988

ASJC Scopus subject areas

  • General

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