A Kinetic Analysis of 6‐[18F]Fluoro‐l‐Dihydroxyphenylalanine Metabolism in the Rat

Paul Cumming, Hiroto Kuwabara, Albert Gjedde

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: A previous study of the metabolism of 6‐[18F]‐fluoro‐l‐3,4‐dihydroxyphenylalanine (FDOPA) in rats pretreated with carbidopa contained information amenable to kinetic analysis. Using these data, tracer transfer coefficients and metabolic rate constants were estimated. After intravenous injection, FDOPA in circulation was O‐methylated (kD0 = 0.055 min−1), and the metabolite (O‐methyl‐FDOPA) escaped from plasma with a rate constant (kM−1) of 0.01 min−1. The initial clearance of FDOPA to striatum (KD1) was 0.07 ml g−1 min−1, and the equilibrium distribution volume (VDe) was 0.67 ml g−1. The initial clearance of O‐methyl‐FDOPA to striatum (KM1) was 0.08 ml g−1 min−1, and the equilibrium distribution volume (VMe) was 0.75 ml g−1. The rate constant of FDOPA decarboxylation (kD3) was 0.17 min−1 in striatum. The elimination of 6‐[18F]fluorodopamine (FDA) from striatum suggested an apparent rate constant for monoamine oxidase activity (k′7) of 0.055 min−1. 6‐[18F]Fluorohomovanillic acid (FHVA) was formed from 6‐[18F]fluoro‐l‐3,4‐dihydroxyphenylacetic acid with a rate constant (k11) of 0.083 min−1, and FHVA was eliminated from striatum (k9) with a rate constant of 0.12 min−1. The steady‐state concentration ratios of FDA and its metabolites were shown to be functions of these rate constants.

Original languageEnglish (US)
Pages (from-to)1675-1682
Number of pages8
JournalJournal of Neurochemistry
Volume63
Issue number5
DOIs
StatePublished - Nov 1994

Keywords

  • 6‐[F]Fluoro‐l‐3,4‐dihydroxyphenylalanine
  • Dopamine
  • Kinetics
  • Metabolism
  • Positron emission tomography
  • Striatum

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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