A human granulocyte-specific antigen characterized by use of monoclonal antibodies

K. M. Skubitz, Y. Zhen, Thomas August

Research output: Contribution to journalArticle

Abstract

Hybridoma cell lines secreting monoclonal antibodies that bind to a surface antigen of human neutrophils have been prepared by fusion of mouse myeloma cells with spleen cells from mice immunized with human neutrophils. Several of the monoclonal antibodies (AHN 1-6) were specific for a neutrophil surface antigen and did not bind lymphocytes, monocytes, red blood cells, platelets, or basophils. All of the grnaulocyte-specific antibodies immunoprecipitated a polypeptide of 145,000 daltons and an isoelectric point of about 4.5 and other heterogeneous polypeptides of 105,000 daltons. These same components were the major lactoperoxidase-labeled proteins precipitated by heperimmune mouse serum. The antibodies were further characterized for binding to several human myeloid leukemia cell lines and cells from patients with myeloid or lymphoid leukemia. All antibodies bound the HL-60, ML1, ML2, ML3, K562, and U937 myeloid leukemia cell lines. None of the antibodies bound the RPMI 6410, Raji, RPMI 8226, MOLT 4, or Daudi lymphoid cell lines. All of the hybridoma cell lines (AHN 1-6) produced IgM antibodies that were cytotoxic.

Original languageEnglish (US)
Pages (from-to)19-26
Number of pages8
JournalBlood
Volume61
Issue number1
StatePublished - 1983
Externally publishedYes

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Granulocytes
Monoclonal Antibodies
Cells
Myeloid Leukemia
Antigens
Cell Line
Antibodies
Neutrophils
Hybridomas
Myeloid Cells
Surface Antigens
Lymphocytes
Lactoperoxidase
Lymphoid Leukemia
U937 Cells
Peptides
Basophils
Isoelectric Point
Immunoglobulin M
Monocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Skubitz, K. M., Zhen, Y., & August, T. (1983). A human granulocyte-specific antigen characterized by use of monoclonal antibodies. Blood, 61(1), 19-26.

A human granulocyte-specific antigen characterized by use of monoclonal antibodies. / Skubitz, K. M.; Zhen, Y.; August, Thomas.

In: Blood, Vol. 61, No. 1, 1983, p. 19-26.

Research output: Contribution to journalArticle

Skubitz, KM, Zhen, Y & August, T 1983, 'A human granulocyte-specific antigen characterized by use of monoclonal antibodies', Blood, vol. 61, no. 1, pp. 19-26.
Skubitz, K. M. ; Zhen, Y. ; August, Thomas. / A human granulocyte-specific antigen characterized by use of monoclonal antibodies. In: Blood. 1983 ; Vol. 61, No. 1. pp. 19-26.
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