A human B cell differentiation antigen selectively expressed on mature B cells identified by a monoclonal antibody (HB-2)

Toru Abo, Alan Landay, Charles M. Balch, Max D. Cooper

Research output: Contribution to journalArticle

Abstract

A monoclonal IgM antibody (HB-2), produced against a membrane antigen on the Raji, B cell line, reacted by indirect immunofluorescence with 2 to 40% of lymphoblasts from the B cell lines, Raji, Daudi, SN-1036, and SB but not with other types of cell lines, including pre-B, myeloid, melanoma, or T cells. HB-2 antibody reacted with 10 ± 3% of normal blood mononuclear cells, and was unreactive with monocytes, granulocytes, platelets, or erythrocytes. Two-color immunofluorescence revealed that HB-2 antigen expression was confined to cells bearing surface Ig. An interesting finding was the fact that 25% of plasmablasts induced by pokeweed mitogen also expressed the HB-2 antigen. However, pre-B and plasma cells from normal bone marrow did not express the HB-2 antigen either on their membrane surface or in their cytoplasm. Analysis of 85 leukemias revealed that the HB-2 antigen was expressed on acute and chronic B cell leukemias and Burkitt's lymphomas, but not on malignacies of pre-B, T, myelocytic, or plasma cells. The results suggest that expression of the HB-2 antigen is confined to mature B cells.

Original languageEnglish (US)
Pages (from-to)253-264
Number of pages12
JournalHuman Immunology
Volume13
Issue number4
DOIs
Publication statusPublished - 1985
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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