A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

Lathiena Manning, Jinal Sheth, Stacey Bridges, Afsoon Saadin, Kamsi Odinammadu, Deborah J Andrew, Susan Spencer, Denise Montell, Michelle Starz-Gaiano

Research output: Contribution to journalArticle

Abstract

Cell migration is essential during animal development. In the Drosophila ovary, the steroid hormone ecdysone coordinates nutrient sensing, growth, and the timing of morphogenesis events including border cell migration. To identify downstream effectors of ecdysone signaling, we profiled gene expression in wild-type follicle cells compared to cells expressing a dominant negative Ecdysone receptor or its coactivator Taiman. Of approximately 400 genes that showed differences in expression, we validated 16 candidate genes for expression in border and centripetal cells, and demonstrated that seven responded to ectopic ecdysone activation by changing their transcriptional levels. We found a requirement for seven putative targets in effective cell migration, including two other nuclear hormone receptors, a calcyphosine-encoding gene, and a prolyl hydroxylase. Thus, we identified multiple new genetic regulators modulated at the level of transcription that allow cells to interpret information from the environment and coordinate cell migration in vivo.

Original languageEnglish (US)
Pages (from-to)56-68
Number of pages13
JournalMechanisms of Development
Volume148
DOIs
StatePublished - Dec 1 2017

Fingerprint

Ecdysone
Cell Movement
Cues
Prolyl Hydroxylases
Gene Expression
Cytoplasmic and Nuclear Receptors
Morphogenesis
Genes
Drosophila
Ovary
Steroids
Hormones
Food
Growth

Keywords

  • Collective cell migration
  • Drosophila
  • Nuclear hormone signaling
  • Oogenesis

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles. / Manning, Lathiena; Sheth, Jinal; Bridges, Stacey; Saadin, Afsoon; Odinammadu, Kamsi; Andrew, Deborah J; Spencer, Susan; Montell, Denise; Starz-Gaiano, Michelle.

In: Mechanisms of Development, Vol. 148, 01.12.2017, p. 56-68.

Research output: Contribution to journalArticle

Manning, L, Sheth, J, Bridges, S, Saadin, A, Odinammadu, K, Andrew, DJ, Spencer, S, Montell, D & Starz-Gaiano, M 2017, 'A hormonal cue promotes timely follicle cell migration by modulating transcription profiles', Mechanisms of Development, vol. 148, pp. 56-68. https://doi.org/10.1016/j.mod.2017.06.003
Manning, Lathiena ; Sheth, Jinal ; Bridges, Stacey ; Saadin, Afsoon ; Odinammadu, Kamsi ; Andrew, Deborah J ; Spencer, Susan ; Montell, Denise ; Starz-Gaiano, Michelle. / A hormonal cue promotes timely follicle cell migration by modulating transcription profiles. In: Mechanisms of Development. 2017 ; Vol. 148. pp. 56-68.
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