TY - JOUR
T1 - A hormonal cue promotes timely follicle cell migration by modulating transcription profiles
AU - Manning, Lathiena
AU - Sheth, Jinal
AU - Bridges, Stacey
AU - Saadin, Afsoon
AU - Odinammadu, Kamsi
AU - Andrew, Deborah
AU - Spencer, Susan
AU - Montell, Denise
AU - Starz-Gaiano, Michelle
N1 - Funding Information:
We thank the Bloomington Drosophila Stock Center for the RNAi and other transgenic flies, the Vienna Drosophila RNAi Center and Harvard TRiP Stock Center for RNAi fly lines, Flybase for genetic and genomic information, and Developmental Studies Hybridoma Bank, K. King-Jones and C. Thummel for antibodies. We thank L. Chodish and the JHMI Microarray core facility for helpful discussions about data analysis. We acknowledge Yvonne Puplampu-Dove and Christy Taylor for help with experiments. We are grateful to Mallika Bhattacharya and Tagide deCarvalho in the Keith Porter Imaging Facility for assistance with imaging the centripetal cells. This project was supported in part by the National Institutes of Health grant R01 013899 (to DA), NIH grant R01 GM73164 (to DM), the National Science Foundation Award IOS-1054422 (to MSG) and Basil O′Connor Starter Scholar Award #5-FY11-477 from the March of Dimes (to MSG).
Publisher Copyright:
© 2017
PY - 2017/12
Y1 - 2017/12
N2 - Cell migration is essential during animal development. In the Drosophila ovary, the steroid hormone ecdysone coordinates nutrient sensing, growth, and the timing of morphogenesis events including border cell migration. To identify downstream effectors of ecdysone signaling, we profiled gene expression in wild-type follicle cells compared to cells expressing a dominant negative Ecdysone receptor or its coactivator Taiman. Of approximately 400 genes that showed differences in expression, we validated 16 candidate genes for expression in border and centripetal cells, and demonstrated that seven responded to ectopic ecdysone activation by changing their transcriptional levels. We found a requirement for seven putative targets in effective cell migration, including two other nuclear hormone receptors, a calcyphosine-encoding gene, and a prolyl hydroxylase. Thus, we identified multiple new genetic regulators modulated at the level of transcription that allow cells to interpret information from the environment and coordinate cell migration in vivo.
AB - Cell migration is essential during animal development. In the Drosophila ovary, the steroid hormone ecdysone coordinates nutrient sensing, growth, and the timing of morphogenesis events including border cell migration. To identify downstream effectors of ecdysone signaling, we profiled gene expression in wild-type follicle cells compared to cells expressing a dominant negative Ecdysone receptor or its coactivator Taiman. Of approximately 400 genes that showed differences in expression, we validated 16 candidate genes for expression in border and centripetal cells, and demonstrated that seven responded to ectopic ecdysone activation by changing their transcriptional levels. We found a requirement for seven putative targets in effective cell migration, including two other nuclear hormone receptors, a calcyphosine-encoding gene, and a prolyl hydroxylase. Thus, we identified multiple new genetic regulators modulated at the level of transcription that allow cells to interpret information from the environment and coordinate cell migration in vivo.
KW - Collective cell migration
KW - Drosophila
KW - Nuclear hormone signaling
KW - Oogenesis
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U2 - 10.1016/j.mod.2017.06.003
DO - 10.1016/j.mod.2017.06.003
M3 - Article
C2 - 28610887
AN - SCOPUS:85035019472
SN - 0925-4773
VL - 148
SP - 56
EP - 68
JO - Mechanisms of Development
JF - Mechanisms of Development
ER -