A high-yield preparative method for isolation of rat liver mitochondria

Ernesto Bustamante, John W. Soper, Peter L. Pedersen

Research output: Contribution to journalArticlepeer-review


A differential centrifugation method for the preparation of rat liver mitochondria is described, which results in final mitochondrial yields of at least 35 to 40 mg of mitochondrial protein per gram wet weight of liver. These mitochondria are shown to be functionally and ultrastructurally intact. They exhibit acceptor control ratios of 6 to 8 and ADP O ratios near 2.0 with succinate as a substrate. In addition, they appear homogeneous by electron microscopy criteria. This method can be used to prepare rat liver mitochondria in high yield on either a large- or a small-scale basis.

Original languageEnglish (US)
Pages (from-to)401-408
Number of pages8
JournalAnalytical biochemistry
Issue number2
StatePublished - Jun 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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