A high-performance liquid chromatographic technique that separates cellular retinol binding protein from cellular retinoic acid binding protein

Fabrizio C. Bonelli, Luigi M De Luca

Research output: Contribution to journalArticle

Abstract

A one-step procedure to detect cellular [3H]retinol and [3H]retinoic acid binding proteins (CRBP and CRABP) from rat testis cytosolic extract was devised. The procedure is based on anion-exchange high-performance liquid chromatography of the cytosolic fraction on columns of Mono Q, which permits elution of CRABP and CRBP at 12 and 22 min, respectively.

Original languageEnglish (US)
Pages (from-to)251-257
Number of pages7
JournalAnalytical Biochemistry
Volume147
Issue number1
DOIs
StatePublished - May 15 1985
Externally publishedYes

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Cellular Retinol-Binding Proteins
Retinoic Acid Receptors
High performance liquid chromatography
Vitamin A
Anions
Rats
Liquids
Testis
High Pressure Liquid Chromatography
Mono Q

Keywords

  • cellular retinoic acid binding protein
  • cellular retinol binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "A one-step procedure to detect cellular [3H]retinol and [3H]retinoic acid binding proteins (CRBP and CRABP) from rat testis cytosolic extract was devised. The procedure is based on anion-exchange high-performance liquid chromatography of the cytosolic fraction on columns of Mono Q, which permits elution of CRABP and CRBP at 12 and 22 min, respectively.",
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