A guide to the development of human corneaorganoids from induced pluripotent stem cells in culture

James W. Foster, Karl J. Wahlin, Shukti Chakravarti

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The cornea is the outermost transparent and refractive barrier surface of the eye necessary for vision. Development of the cornea involves the coordinated production of extracellular matrix, epithelial differentiation, and endothelial cell expansion to produce a highly transparent tissue. Here we describe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for multiple downstream applications which are currently unattainable using traditional in vitro techniques.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages51-58
Number of pages8
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2145
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cornea
  • Corneal epithelium
  • Corneal stroma
  • Organoids
  • iPSC

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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