TY - JOUR
T1 - A glucose sensor role for glucokinase in anterior pituitary cells
AU - Zelent, Dorothy
AU - Golson, Maria L.
AU - Koeberlein, Brigitte
AU - Quintens, Roel
AU - Van Lommel, Leentje
AU - Buettger, Carol
AU - Weik-Collins, Heather
AU - Taub, Rebecca
AU - Grimsby, Joseph
AU - Schuit, Frans
AU - Kaestner, Klaus H.
AU - Matschinsky, Franz M.
PY - 2006/7
Y1 - 2006/7
N2 - Enzymatic activity of glucokinase was demonstrated, quantitated, and characterized kinetically in rat and mouse pituitary extracts using a highly specific and sensitive spectrometric assay. A previously proposed hypothesis that the glucokinase gene might be expressed in the pituitary corticotrophic cells was therefore reexamined using mRNA in situ hybridization and immunohistochemical techniques. No evidence was found that corticotrophs are glucokinase positive, and the identity of glucokinase-expressing cells remains to be determined. The findings do, however, suggest a novel hypothesis that a critical subgroup of anterior pituitary cells might function as glucose sensor cells and that direct fuel regulation of such cells may modify the classical indirect neuroendocrine pathways that are known to control hormone secretion from anterior pituitary cells.
AB - Enzymatic activity of glucokinase was demonstrated, quantitated, and characterized kinetically in rat and mouse pituitary extracts using a highly specific and sensitive spectrometric assay. A previously proposed hypothesis that the glucokinase gene might be expressed in the pituitary corticotrophic cells was therefore reexamined using mRNA in situ hybridization and immunohistochemical techniques. No evidence was found that corticotrophs are glucokinase positive, and the identity of glucokinase-expressing cells remains to be determined. The findings do, however, suggest a novel hypothesis that a critical subgroup of anterior pituitary cells might function as glucose sensor cells and that direct fuel regulation of such cells may modify the classical indirect neuroendocrine pathways that are known to control hormone secretion from anterior pituitary cells.
KW - GKA, glucokinase activator
KW - GST, glutathione S-transferase
KW - POMC, proopiomelanocortin
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U2 - 10.2337/db06-0151
DO - 10.2337/db06-0151
M3 - Article
C2 - 16804059
AN - SCOPUS:33747033203
SN - 0012-1797
VL - 55
SP - 1923
EP - 1929
JO - Diabetes
JF - Diabetes
IS - 7
ER -