A genomic-scale view of the camp response element-enhancer decoy: A tumor target-based genetic tool

Yee Sook Cho, Meyoung Kon Kim, Chris Cheadle, Catherine Neary, Yun Gyu Park, Kevin G. Becker, Yoon S. Cho-Chung

Research output: Contribution to journalArticle

Abstract

Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.

Original languageEnglish (US)
Pages (from-to)15626-15631
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number24
DOIs
StatePublished - Nov 26 2002
Externally publishedYes

Fingerprint

Response Elements
Oligodeoxyribonucleotides
Neoplasms
Up-Regulation
Transcription Factor AP-2
Growth
Genes
Microarray Analysis
Multigene Family
Oligonucleotide Array Sequence Analysis
Tumor Cell Line
Nude Mice
Cell Differentiation
Transcription Factors
Cell Proliferation
Liver
DNA

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A genomic-scale view of the camp response element-enhancer decoy : A tumor target-based genetic tool. / Cho, Yee Sook; Kim, Meyoung Kon; Cheadle, Chris; Neary, Catherine; Park, Yun Gyu; Becker, Kevin G.; Cho-Chung, Yoon S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 24, 26.11.2002, p. 15626-15631.

Research output: Contribution to journalArticle

Cho, Yee Sook ; Kim, Meyoung Kon ; Cheadle, Chris ; Neary, Catherine ; Park, Yun Gyu ; Becker, Kevin G. ; Cho-Chung, Yoon S. / A genomic-scale view of the camp response element-enhancer decoy : A tumor target-based genetic tool. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 24. pp. 15626-15631.
@article{482f6476b8724f43a23165bf7a72fc13,
title = "A genomic-scale view of the camp response element-enhancer decoy: A tumor target-based genetic tool",
abstract = "Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.",
author = "Cho, {Yee Sook} and Kim, {Meyoung Kon} and Chris Cheadle and Catherine Neary and Park, {Yun Gyu} and Becker, {Kevin G.} and Cho-Chung, {Yoon S.}",
year = "2002",
month = "11",
day = "26",
doi = "10.1073/pnas.242617799",
language = "English (US)",
volume = "99",
pages = "15626--15631",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "24",

}

TY - JOUR

T1 - A genomic-scale view of the camp response element-enhancer decoy

T2 - A tumor target-based genetic tool

AU - Cho, Yee Sook

AU - Kim, Meyoung Kon

AU - Cheadle, Chris

AU - Neary, Catherine

AU - Park, Yun Gyu

AU - Becker, Kevin G.

AU - Cho-Chung, Yoon S.

PY - 2002/11/26

Y1 - 2002/11/26

N2 - Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.

AB - Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.

UR - http://www.scopus.com/inward/record.url?scp=0037180546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037180546&partnerID=8YFLogxK

U2 - 10.1073/pnas.242617799

DO - 10.1073/pnas.242617799

M3 - Article

C2 - 12438686

AN - SCOPUS:0037180546

VL - 99

SP - 15626

EP - 15631

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 24

ER -