A genomewide screen for components of the RNAi pathway in Drosophila cultured cells

Silke Dorner, Lawrence Lum, Michelle Kim, Renato Paro, Philip A. Beachy, Rachel Green

Research output: Contribution to journalArticle

Abstract

Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG10883).

Original languageEnglish (US)
Pages (from-to)11880-11885
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number32
DOIs
StatePublished - Aug 8 2006

Keywords

  • Posttranscriptional silencing
  • RNAi screen
  • dsRNA
  • siRNA
  • siRNA pathway

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'A genomewide screen for components of the RNAi pathway in Drosophila cultured cells'. Together they form a unique fingerprint.

  • Cite this