A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila

Tingting Xie, Margaret C.W. Ho, Qili Liu, Wakako Horiuchi, Chun Chieh Lin, Darya Task, Haojiang Luan, Benjamin H. White, Christopher J Potter, Mark Wu

Research output: Contribution to journalArticle

Abstract

The neuromodulator dopamine (DA) plays a key role in motor control, motivated behaviors, and higher-order cognitive processes. Dissecting how these DA neural networks tune the activity of local neural circuits to regulate behavior requires tools for manipulating small groups of DA neurons. To address this need, we assembled a genetic toolkit that allows for an exquisite level of control over the DA neural network in Drosophila. To further refine targeting of specific DA neurons, we also created reagents that allow for the conversion of any existing GAL4 line into Split GAL4 or GAL80 lines. We demonstrated how this toolkit can be used with recently developed computational methods to rapidly generate additional reagents for manipulating small subsets or individual DA neurons. Finally, we used the toolkit to reveal a dynamic interaction between a small subset of DA neurons and rearing conditions in a social space behavioral assay. The rapid analysis of how dopaminergic circuits regulate behavior is limited by the genetic tools available to target and manipulate small numbers of these neurons. Xie et al. present genetic tools in Drosophila that allow rational targeting of sparse dopaminergic neuronal subsets and selective knockdown of dopamine signaling.

Original languageEnglish (US)
Pages (from-to)652-665
Number of pages14
JournalCell Reports
Volume23
Issue number2
DOIs
StatePublished - Apr 10 2018

Fingerprint

Dopaminergic Neurons
Drosophila
Dopamine
Networks (circuits)
Neurons
Behavior Control
Neurotransmitter Agents
Neural networks
Computational methods
Assays

Keywords

  • behavior
  • dopamine
  • Drosophila
  • genetics
  • neural circuits
  • neuromodulation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Xie, T., Ho, M. C. W., Liu, Q., Horiuchi, W., Lin, C. C., Task, D., ... Wu, M. (2018). A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila. Cell Reports, 23(2), 652-665. https://doi.org/10.1016/j.celrep.2018.03.068

A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila. / Xie, Tingting; Ho, Margaret C.W.; Liu, Qili; Horiuchi, Wakako; Lin, Chun Chieh; Task, Darya; Luan, Haojiang; White, Benjamin H.; Potter, Christopher J; Wu, Mark.

In: Cell Reports, Vol. 23, No. 2, 10.04.2018, p. 652-665.

Research output: Contribution to journalArticle

Xie, T, Ho, MCW, Liu, Q, Horiuchi, W, Lin, CC, Task, D, Luan, H, White, BH, Potter, CJ & Wu, M 2018, 'A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila', Cell Reports, vol. 23, no. 2, pp. 652-665. https://doi.org/10.1016/j.celrep.2018.03.068
Xie T, Ho MCW, Liu Q, Horiuchi W, Lin CC, Task D et al. A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila. Cell Reports. 2018 Apr 10;23(2):652-665. https://doi.org/10.1016/j.celrep.2018.03.068
Xie, Tingting ; Ho, Margaret C.W. ; Liu, Qili ; Horiuchi, Wakako ; Lin, Chun Chieh ; Task, Darya ; Luan, Haojiang ; White, Benjamin H. ; Potter, Christopher J ; Wu, Mark. / A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila. In: Cell Reports. 2018 ; Vol. 23, No. 2. pp. 652-665.
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