A genetic analysis of glucocorticoid receptor signaling: Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae

R. Sitcheran, R. Emter, Anastasia Kralli, K. R. Yamamoto

Research output: Contribution to journalArticle

Abstract

To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.

Original languageEnglish (US)
Pages (from-to)963-972
Number of pages10
JournalGenetics
Volume156
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

Glucocorticoid Receptors
Saccharomyces cerevisiae
Signal Transduction
Ligands
Ergosterol
Biosynthetic Pathways
Methyltransferases
Dexamethasone
Glucocorticoids
Proteins
Yeasts
Hormones
Genes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

A genetic analysis of glucocorticoid receptor signaling : Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae. / Sitcheran, R.; Emter, R.; Kralli, Anastasia; Yamamoto, K. R.

In: Genetics, Vol. 156, No. 3, 2000, p. 963-972.

Research output: Contribution to journalArticle

@article{0fc9f4eb99934fd5a286ded035a2ab86,
title = "A genetic analysis of glucocorticoid receptor signaling: Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae",
abstract = "To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.",
author = "R. Sitcheran and R. Emter and Anastasia Kralli and Yamamoto, {K. R.}",
year = "2000",
language = "English (US)",
volume = "156",
pages = "963--972",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "3",

}

TY - JOUR

T1 - A genetic analysis of glucocorticoid receptor signaling

T2 - Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae

AU - Sitcheran, R.

AU - Emter, R.

AU - Kralli, Anastasia

AU - Yamamoto, K. R.

PY - 2000

Y1 - 2000

N2 - To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.

AB - To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.

UR - http://www.scopus.com/inward/record.url?scp=0033761504&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033761504&partnerID=8YFLogxK

M3 - Article

C2 - 11063677

AN - SCOPUS:0033761504

VL - 156

SP - 963

EP - 972

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 3

ER -