A genetic analysis of glucocorticoid receptor signaling: Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae

R. Sitcheran, R. Emter, A. Kralli, K. R. Yamamoto

Research output: Contribution to journalArticlepeer-review

Abstract

To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.

Original languageEnglish (US)
Pages (from-to)963-972
Number of pages10
JournalGenetics
Volume156
Issue number3
StatePublished - Nov 20 2000
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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