A gap at a unique location in newly replicated kinetoplast DNA minicircles from Trypanosoma equiperdum

J. M. Ntambi, P. T. Englund

Research output: Contribution to journalArticle

Abstract

Kinetoplast DNA is a network containing thousands of interlocked minicircles. The minicircles replicate as free molecules after release from the network, and their progeny are then reattached. In Trypanosoma equiperdum, some of the newly replicated minicircles which have recatenated to the network contain a single gap. This gap is about 10 nucleotides in size and it is in the newly synthesized strand. Based on several criteria (S1 nuclease digestion, denaturing polyacrylamide gel analysis and DNA sequencing), the gap residues at a unique site on the molecule. This site overlaps the sequence GGGGTTGGTGTAA, which is the only common sequence found in all minicircles, from several different species, which have been examined.

Original languageEnglish (US)
Pages (from-to)5574-5579
Number of pages6
JournalJournal of Biological Chemistry
Volume260
Issue number9
StatePublished - 1985

Fingerprint

Kinetoplast DNA
Trypanosoma
DNA Sequence Analysis
Digestion
Nucleotides
Molecules
DNA
polyacrylamide gels

ASJC Scopus subject areas

  • Biochemistry

Cite this

A gap at a unique location in newly replicated kinetoplast DNA minicircles from Trypanosoma equiperdum. / Ntambi, J. M.; Englund, P. T.

In: Journal of Biological Chemistry, Vol. 260, No. 9, 1985, p. 5574-5579.

Research output: Contribution to journalArticle

@article{86be3addeead45f8a953000d18c16eec,
title = "A gap at a unique location in newly replicated kinetoplast DNA minicircles from Trypanosoma equiperdum",
abstract = "Kinetoplast DNA is a network containing thousands of interlocked minicircles. The minicircles replicate as free molecules after release from the network, and their progeny are then reattached. In Trypanosoma equiperdum, some of the newly replicated minicircles which have recatenated to the network contain a single gap. This gap is about 10 nucleotides in size and it is in the newly synthesized strand. Based on several criteria (S1 nuclease digestion, denaturing polyacrylamide gel analysis and DNA sequencing), the gap residues at a unique site on the molecule. This site overlaps the sequence GGGGTTGGTGTAA, which is the only common sequence found in all minicircles, from several different species, which have been examined.",
author = "Ntambi, {J. M.} and Englund, {P. T.}",
year = "1985",
language = "English (US)",
volume = "260",
pages = "5574--5579",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "9",

}

TY - JOUR

T1 - A gap at a unique location in newly replicated kinetoplast DNA minicircles from Trypanosoma equiperdum

AU - Ntambi, J. M.

AU - Englund, P. T.

PY - 1985

Y1 - 1985

N2 - Kinetoplast DNA is a network containing thousands of interlocked minicircles. The minicircles replicate as free molecules after release from the network, and their progeny are then reattached. In Trypanosoma equiperdum, some of the newly replicated minicircles which have recatenated to the network contain a single gap. This gap is about 10 nucleotides in size and it is in the newly synthesized strand. Based on several criteria (S1 nuclease digestion, denaturing polyacrylamide gel analysis and DNA sequencing), the gap residues at a unique site on the molecule. This site overlaps the sequence GGGGTTGGTGTAA, which is the only common sequence found in all minicircles, from several different species, which have been examined.

AB - Kinetoplast DNA is a network containing thousands of interlocked minicircles. The minicircles replicate as free molecules after release from the network, and their progeny are then reattached. In Trypanosoma equiperdum, some of the newly replicated minicircles which have recatenated to the network contain a single gap. This gap is about 10 nucleotides in size and it is in the newly synthesized strand. Based on several criteria (S1 nuclease digestion, denaturing polyacrylamide gel analysis and DNA sequencing), the gap residues at a unique site on the molecule. This site overlaps the sequence GGGGTTGGTGTAA, which is the only common sequence found in all minicircles, from several different species, which have been examined.

UR - http://www.scopus.com/inward/record.url?scp=0022262659&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022262659&partnerID=8YFLogxK

M3 - Article

C2 - 2985601

AN - SCOPUS:0022262659

VL - 260

SP - 5574

EP - 5579

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 9

ER -