A GAL80 collection to inhibit GAL4 transgenes in drosophila olfactory sensory neurons

Jessica Eliason, Ali Afify, Christopher Potter, Ichiro Matsumura

Research output: Contribution to journalArticle

Abstract

Fruit flies recognize hundreds of ecologically relevant odors and respond appropriately to them. The complexity, redundancy and interconnectedness of the olfactory machinery complicate efforts to pinpoint the functional contributions of any component neuron or receptor to behavior. Some contributions can only be elucidated in flies that carry multiple mutations and transgenes, but the production of such flies is currently labor-intensive and time-consuming. Here, we describe a set of transgenic flies that express the Saccharomyces cerevisiae GAL80 in specific olfactory sensory neurons (OrX-GAL80s). The GAL80s effectively and specifically subtract the activities of GAL4-driven transgenes that impart anatomical and physiological phenotypes. OrX-GAL80s can allow researchers to efficiently activate only one or a few types of functional neurons in an otherwise nonfunctional olfactory background. Such experiments will improve our understanding of the mechanistic connections between odorant inputs and behavioral outputs at the resolution of only a few functional neurons.

Original languageEnglish (US)
Pages (from-to)3661-3668
Number of pages8
JournalG3: Genes, Genomes, Genetics
Volume8
Issue number11
DOIs
StatePublished - Nov 1 2018

Fingerprint

Olfactory Receptor Neurons
Transgenes
Diptera
Drosophila
Neurons
Saccharomyces cerevisiae
Fruit
Research Personnel
Phenotype
Mutation
Odorants

Keywords

  • Drosophila
  • GAL80
  • Odorant
  • Olfaction
  • Receptor
  • Sensory neurons

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

A GAL80 collection to inhibit GAL4 transgenes in drosophila olfactory sensory neurons. / Eliason, Jessica; Afify, Ali; Potter, Christopher; Matsumura, Ichiro.

In: G3: Genes, Genomes, Genetics, Vol. 8, No. 11, 01.11.2018, p. 3661-3668.

Research output: Contribution to journalArticle

Eliason, Jessica ; Afify, Ali ; Potter, Christopher ; Matsumura, Ichiro. / A GAL80 collection to inhibit GAL4 transgenes in drosophila olfactory sensory neurons. In: G3: Genes, Genomes, Genetics. 2018 ; Vol. 8, No. 11. pp. 3661-3668.
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